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Hesperidin

Manufactured by Santa Cruz Biotechnology
Sourced in Germany, United States

Hesperidin is a flavonoid compound derived from citrus fruits. It is a natural product found in high concentrations in the peel and white pulp of citrus fruits such as oranges, lemons, and grapefruits. Hesperidin is a key component in various laboratory applications and research studies.

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3 protocols using hesperidin

1

Hesperidin Administration in Animal Study

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Hesperidin (Hesperetin 7-rhamnoglucoside; Cirantin; Hesperetin-7-rutinoside, C28H34O15, MW: 610.56, purity ≥ 95%, CAS Number: 520-26-3) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, United States of America). The compound was dissolved in a saline solution containing 0.5% Tween-80. Hesperidin was administered intraperitoneally (i.p.) at doses of 100 mg/kg. Drug administration was performed 60 minutes before the surgical procedure. Hesperidin doses were preferred based on previous experiments13 (link).
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2

Mineral Ion Balance Regulation

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Hesperidin and gallic acid were obtained from SantaCruz Biotechnology Inc, Heidelberg Germany. Kits for evaluating serum levels of potassium, calcium, and magnesium were obtained from Atlas Medical, Blankenfelde-Mahlow, Berlin, Germany. Kits for estimating serum levels of phosphate, chloride, and bicarbonate were purchased from Teco Diagnostics, Anaheim, USA. ELISA kits for evaluating Na+/K+ ATPase and Ca2+/Mg2+ ATPase were purchased from MyBiosource, Inc, San Diego, USA. All other chemicals and reagents used were of analytical grade.
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3

Hesperidin effects on cell death

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All cell culture reagents were purchased from GIBCO Life Technologies (Thermo Fisher Scientific, USA). Hesperidin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The WST-1, Cell Death Detection ELISA PLUS and High Pure RNA Isolation kits were purchased from Roche Life Sciences (Germany). The CytoTox 96R Non-Radioactive Cytotoxicity Assay kit was purchased from Promega Corporation (Madison, WI, USA). The FITC Annexin V Apoptosis Detection kit was purchased from BD Biosciences (Franklin Lakes, NJ, USA). The Caspase-3 colorimetric kit was purchased from BioVision Research Products (Milpitas, CA, USA). The JC-1 Mitochondrial Membrane Potential Detection kit was purchased from Cayman Chemical (Ann Arbor, MI, USA). Human whole genome gene expression profiling was performed using Illumina Human HT-12v4 beadchips and microarray reagents (San Diego, CA, USA).
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