Cyclin d1 sc 717

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Cyclin D1(sc-717) is a primary antibody used in immunohistochemistry, western blotting, and other applications to detect the presence of the Cyclin D1 protein. Cyclin D1 is a cell cycle regulatory protein that plays a crucial role in the G1/S transition phase of the cell cycle.

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Lab products found in correlation

Anti actin, by Merck Group (1 mentions) Sp2509, by Selleck Chemicals (1 mentions) Enzalutamide, by Selleck Chemicals (1 mentions) β actin a1978, by Merck Group (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Ab103590, by Abcam (1 mentions) Upa sc 14019, by Santa Cruz Biotechnology (1 mentions) Mmp 2 sc 53630, by Santa Cruz Biotechnology (1 mentions) Antibodies against p21 sc 397, by Santa Cruz Biotechnology (1 mentions) Mmp 9 sc 6840, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated anti mouse and anti rabbit secondary antibodies, by Promega (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)

3 protocols using cyclin d1 sc 717

Investigating AR and EMT in Cancer

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Validated AR shRNA lenti-virus expression plasmid and the AR ectopic expression plasmid were kindly provided by Dr. Huang (Mayo Clinic). Anti-Actin was from Sigma-Aldrich (St. Louis, MO); antibodies for AR (sc-7305), Twist (sc-15393), BRD4 (sc-48772), p21(sc-397,) and Cyclin D1(sc-717) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-LSD1 (4064), Anti-cleaved PARP (5625) and Anti-Snail (4719) were from Cell Signaling Technology (Danvers, MA). JQ1, SP-2509 and enzalutamide were from Selleckchem (Houston, TX).

Wang J., Yu Q., Qiu Z., Dai T., Wang S., Yang X., Evers B.M, & Wu Y. (2020). The combined effect of epigenetic inhibitors for LSD1 and BRD4 alters prostate cancer growth and invasion. Aging (Albany NY), 12(1), 397-415.

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Protein Expression Analysis by Western Blot

Cited 1 time

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Western blot analysis was performed on cell and tissue lysates as described.^{62 (link)} Antibodies used in this study were as follows: phospho-STAT3 (Y705) (no. 9145) and STAT3 (no. 4904) from Cell Signaling (Danvers, MA), Cyclin D1 (sc-717) from Santa Cruz Biotechnologies (Santa Cruz, CA), β-actin (A1978) from Sigma-Aldrich (St. Louis, MO, USA), and GAPDH (no. 3783) from ProsciΨ (Poway, California, USA).

Hou S., Yi Y.W., Jin Kang H., Zhang L., Kim H.J., Kong Y., Liu Y., Wang K., Kong H.S., Grindrod S., Bae I, & Brown M.L. (2014). Novel Carbazole Inhibits Phospho-STAT3 through Induction of Protein–Tyrosine Phosphatase PTPN6. Journal of medicinal chemistry, 57(15), 6342-6353.

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Antibody-based Protein Quantification Assay

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Antibody against ESM1 (ab103590) was purchased from Abcam (Cambridge. MA). Antibodies against p21 (sc-397; 1:1000), MMP-2 (sc-53630; 1:1000), MMP-9 (sc-6840; 1:1000), uPA (sc-14019; 1:1000), cyclin D1 (sc717; 1:1000), and β-actin (sc-47778; 1:2000) were purchased from Santa Cruz Biotechnology (Dallas, Texas). Horseradish peroxidase conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from Promega (Madison, WI). MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] was purchased from Sigma (St. Louis, MO). Recombinant Human TIMP-1 Protein was purchased from R&D Systems, Inc (Minneapolis, MN). Antibody against TIMP-1(#8946; 1:1000) was purchased from Cell Signaling Technology Inc. (Beverly, MA, USA).

Chen C.M., Lin C.L., Chiou H.L., Hsieh S.C., Lin C.L., Cheng C.W., Hung C.H., Tsai J.P, & Hsieh Y.H. (2017). Loss of endothelial cell-specific molecule 1 promotes the tumorigenicity and metastasis of prostate cancer cells through regulation of the TIMP-1/MMP-9 expression. Oncotarget, 8(8), 13886-13897.

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