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Dm4000 upright microscopy

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The Leica DM4000 is an upright microscopy system designed for a wide range of applications. It features advanced optics, a motorized nosepiece, and a high-resolution camera interface to capture detailed images and videos. The DM4000 provides a versatile platform for various scientific and research purposes, enabling users to explore specimens with precision and clarity.

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Lab products found in correlation

Alexa 488 and 568, by Thermo Fisher Scientific (3 mentions) Olig2, by R&D Systems (3 mentions) Tom20, by Santa Cruz Biotechnology (2 mentions) Phospho drp1s616, by Cell Signaling Technology (2 mentions) Titan confocal, by Leica (2 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Scanr analysis software, by Olympus (1 mentions) Sybr gold dna stain, by Thermo Fisher Scientific (1 mentions) Cdc20, by Santa Cruz Biotechnology (1 mentions)

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DM4000 (200 citations) Upright microscopy (2 citations)

5 protocols using dm4000 upright microscopy

1

Quantification of RPA in GBM Cells

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Detection of total RPA (1 : 250; Millipore) and was performed as described previously.36 (link) GBM cells were grown on coverslips and fixed 24 h after plating. Cells were then immunostained for RPA. Secondary detection was accomplished using Alexa Fluor 488 goat anti-mouse IgG (Invitrogen). Nuclei were counterstained with DAPI. Images were taken using a Leica DM4000 Upright microscopy (Leica). Slides were scanned with the ScanR screening station and data acquired analyzed using ScanR analysis software (Olympus, Hamburg, Germany). Signal was normalized to DAPI per cell.
Rivera M., Wu Q., Hamerlik P., Hjelmeland A.B., Bao S, & Rich J.N. (2015). Acquisition of meiotic DNA repair regulators maintain genome stability in glioblastoma. Cell Death & Disease, 6(4), e1732-.
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2

Alkaline Comet Assay for DNA Damage

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DNA damage repair was assessed by single-cell electrophoresis assay under alkaline conditions as previously described53 (link) (Trevigen, Gaithersburg, MD, USA). Briefly, cells were resuspended to 1 × 105 cells/ml, mixed with agarose (1 : 10) and plated on comet slides. Cells were lysed and placed in alkali unwinding solution. After electrophoresis, DNA was stained with sybr gold DNA stain (Invitrogen). Images were taken using a Leica DM4000 Upright microscopy (Leica, Buffalo Grove, IL, USA). Analysis was done using CASPLab software (CASPLab.com).40 (link)
Rivera M., Wu Q., Hamerlik P., Hjelmeland A.B., Bao S, & Rich J.N. (2015). Acquisition of meiotic DNA repair regulators maintain genome stability in glioblastoma. Cell Death & Disease, 6(4), e1732-.
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3

Imaging Xenografted Brain Tissue

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Cells or 10 µm thick slices of xenografted brain tissue were fixed in 4% paraformaldehyde and immunolabeled using the following antibodies: DRP1 (BD Biosystems), phospho-DRP1S616 (Cell Signaling, Danvers, MA), TOM20 (Santa Cruz Biotechnology, Santa Cruz, CA), OLIG2 (R&D Systems), and SOX2 (R&D Systems). Primary antibodies were incubated overnight at 4°C, followed by species appropriate secondary antibodies (Alexa 488 and 568; Invitrogen Molecular Probes, Eugene, OR) with incubation for 1 hour. Nuclei were stained with DAPI, and slides were then mounted using Fluoromount (Calbiochem, San Diego, CA). Images were taken using a Leica Titan confocal or DM4000 Upright microscopy.
Xie Q., Wu Q., Horbinski C.M., Flavahan W.A., Yang K., Zhou W., Dombrowski S.M., Huang Z., Fang X., Shi Y., Ferguson A.N., Kashatus D.F., Bao S, & Rich J.N. (2015). Mitochondrial Control by DRP1 in Brain Tumor Initiating Cells. Nature neuroscience, 18(4), 501-510.
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4

Imaging Xenografted Brain Tissue

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Cells or 10 µm thick slices of xenografted brain tissue were fixed in 4% paraformaldehyde and immunolabeled using the following antibodies: DRP1 (BD Biosystems), phospho-DRP1S616 (Cell Signaling, Danvers, MA), TOM20 (Santa Cruz Biotechnology, Santa Cruz, CA), OLIG2 (R&D Systems), and SOX2 (R&D Systems). Primary antibodies were incubated overnight at 4°C, followed by species appropriate secondary antibodies (Alexa 488 and 568; Invitrogen Molecular Probes, Eugene, OR) with incubation for 1 hour. Nuclei were stained with DAPI, and slides were then mounted using Fluoromount (Calbiochem, San Diego, CA). Images were taken using a Leica Titan confocal or DM4000 Upright microscopy.
Xie Q., Wu Q., Horbinski C.M., Flavahan W.A., Yang K., Zhou W., Dombrowski S.M., Huang Z., Fang X., Shi Y., Ferguson A.N., Kashatus D.F., Bao S, & Rich J.N. (2015). Mitochondrial Control by DRP1 in Brain Tumor Initiating Cells. Nature neuroscience, 18(4), 501-510.
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5

Immunostaining of Xenografted Brain Tissue

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Cells or 10 μm thick slices of xenografted brain tissue were fixed in 4% paraformaldehyde and immunolabeled using the following antibodies: CDC20 (Santa Cruz Biotechnology, Santa Cruz, CA), OLIG2 (R&D Systems), and SOX2 (R&D Systems). Primary antibodies were incubated overnight at 4°C, followed by species appropriate secondary antibodies (Alexa 488 and 568; Invitrogen Molecular Probes, Eugene, OR) with incubation for 1 hour. Nuclei were stained with DAPI, and slides were then mounted using Fluoromount (Calbiochem, San Diego, CA). Images were taken using a Leica DM4000 Upright microscopy.
Xie Q., Wu Q., Mack S.C., Yang K., Kim L., Hubert C.G., Flavahan W.A., Chu C., Bao S, & Rich J.N. (2015). CDC20 maintains tumor initiating cells. Oncotarget, 6(15), 13241-13254.
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