Cytoplasmic and nuclear protein extraction and protein quantification was performed according to the manufacturer’s recommendations (CelLytic™ NuCLEAR™ Extraction Kit, MERCK and Pierce™ BCA Protein Assay Kit, ThermoFisher respectively). Proteins were separated by PAGE and transferred following standard protocols62 (link). Membranes were first probed using the following primary antibodies: anti-HIF1α (BD610958, clone 54 from Bioscience; 1/500), anti-Lamin B1 (sc374015, Santa Cruz; 1/500) or anti-α-tubulin (DM1A, Sigma; 1/4000). Next, membranes were incubated with secondary IRDye680 and IRDye800 antibodies (respectively 926-68021 and 926-32211, Licor; 1/15000). Bands were detected with the Odyssey Infrared Imaging System (Licor) and quantified using ImageStudio Lite 5.2 software (Licor).
Anti lamin b1 sc374015
Manufactured by Santa Cruz Biotechnology
Anti-Lamin B1 (sc-374015) is a laboratory reagent used for the detection and study of Lamin B1 protein. Lamin B1 is a structural protein that is a component of the nuclear lamina, which provides mechanical support and organization to the cell nucleus. This antibody can be used in various immunological techniques, such as Western blotting and immunofluorescence, to investigate the expression and localization of Lamin B1 in biological samples.
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Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Image studio lite 5, by LI COR (1 mentions)
Anti α tubulin dm1a, by Merck Group (1 mentions)
Cellytic nuclear extraction kit, by Merck Group (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Anti relb, by Cell Signaling Technology (1 mentions)
Anti p100 p52, by Merck Group (1 mentions)
Anti cdk4 sc 260, by Santa Cruz Biotechnology (1 mentions)
Anti p21 sc 397, by Santa Cruz Biotechnology (1 mentions)
Anti p130, by BD (1 mentions)
Anti p50, by Cell Signaling Technology (1 mentions)
Anti ezh2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
2 protocols using anti lamin b1 sc374015
1
Protein Extraction and Quantification for Western Blot
2
Antibody Panel for Cell Signaling Analysis
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Antibodies used were: anti-EZH2 (3147S Cell Signaling), anti-p52/p100 (05-361 Millipore), anti-RelB (4954S Cell Signaling), anti-p53 (DO-1 sc-126 Santa Cruz), anti-β- Actin (A5441, Sigma), anti-p21 (sc-397 Santa Cruz), anti-Rb (sc-50 Santa Cruz), anti- Cdk4 (sc-260 Santa Cruz), anti-DEK (610948 BD transduction Laboratories), anti-Bcl3 (PA1-41087 Pierce), anti-Lamin B1 (sc-374015 Santa Cruz), anti-p50 (3035S Cell Signaling), anti-MDM2 (OP46 Calbiochem), anti-p130 (610261 BD transduction Laboratories), anti-p107 (sc-318 Santa Cruz), anti-PSMA5 (2457S Cell Signaling), anti-p14ARF (14PO2 Calbiochem), anti-p16INK4a (sc-56330 Santa Cruz), anti MnSOD (sc-133134 Santa Cruz). Phospho-antibodies used were S15-p53 (9284S Cell Signaling) and S780- Rb (8180S Cell Signaling). Lymphotoxin β receptor agonist antibody (anti-HuLTβR:Fc Ab) was a kind gift of Prof. Carl Ware (Sanford/Burnham Medical Research Institute) [64] (link).
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