All consumables were of ultra-pure or electrophoresis grade. Electrophoresis apparatuses, ReadyStripTM IPG Strips (7 cm, pH 3–10 non-linear), Bio-LyteTM carrier ampholytes, and Precision Plus ProteinTM Unstained Standards (10–250 kDa) were supplied by Bio-Rad Laboratories (Hercules, CA, USA). Isolated protein standards (Bovine Serum Albumin (BSA), Chicken Egg Albumin (CEA) and Chicken Egg Lysozyme (CEL)), and 2-hydroxyethyl disulfide (HED) were supplied by Sigma-Aldrich (St. Louis, MO, USA). Acrylamide/bis-acrylamide (40%, 37.5:1) solution, components of the protease inhibitor (PI) cocktail, thiourea, urea, N,N,N’,N’-tetramethylethylenediamine, sodium n-dodecyl sulfate, ammonium sulfate, glycerol, phosphoric acid, ammonium peroxydisulfate, and tributylphosphine (TBP) were obtained from Alfa Aesar (Haverhill, MA, USA). Sodium chloride, methanol, mineral oil, and dithiothreitol (DTT) were from Thermo Fischer Scientific (Waltham, MA, USA). Acrylamide and citric acid were obtained from BioShop Canada Inc (Burlington, ON, Canada). Coomassie Brilliant Blue G-250, CHAPS, tris hydrochloride, Tris-Glycine-SDS powder, and low-melting agarose were supplied by VWR (Radnor, PA, USA). Double glass-distilled water (ddH2O) was used throughout.
Sodium n dodecyl sulfate
Manufactured by Thermo Fisher Scientific
Sourced in United States
Sodium n-dodecyl sulfate is a commonly used anionic surfactant. It is a white to yellowish crystalline powder that is soluble in water and has a characteristic odor.
Automatically generated - may contain errors
Lab products found in correlation
Copper 1 iodide, by Merck Group (2 mentions)
2 2 6 6 tetramethylpiperidine, by Merck Group (2 mentions)
Triethylamine, by Merck Group (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Urea, by Thermo Fisher Scientific (1 mentions)
Thiourea, by Thermo Fisher Scientific (1 mentions)
Ammonium sulfate, by Thermo Fisher Scientific (1 mentions)
Phosphoric acid, by Thermo Fisher Scientific (1 mentions)
Mineral oil, by Thermo Fisher Scientific (1 mentions)
Glycerol, by Thermo Fisher Scientific (1 mentions)
Readystriptm ipg strips, by Bio-Rad (1 mentions)
Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions)
Ammonium peroxydisulfate, by Thermo Fisher Scientific (1 mentions)
Tris hydrochloride, by Avantor (1 mentions)
2 hydroxyethyl disulfide, by Merck Group (1 mentions)
Precision plus proteintm unstained standard, by Bio-Rad (1 mentions)
Coomassie brilliant blue g 250, by Avantor (1 mentions)
N n n n tetramethylethylenediamine, by Thermo Fisher Scientific (1 mentions)
Chicken egg lysozyme, by Merck Group (1 mentions)
4 protocols using sodium n dodecyl sulfate
1
Two-Dimensional Gel Electrophoresis Protocol
2
Synthesis and Characterization of Dye-Loaded Silica Nanoparticles
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Tetraethyl orthosilicate (TEOS, 98%), dopamine·hydrochloride, tris (hydroxymethyl) aminomethane (Tris) and potassium persulfate (99+%) were purchased from Sigma-Aldrich. Sudan I, Sudan Blue II and Alizarin Yellow GG were purchased from Tokyo Chemical Industry Co., Ltd. and ethyl alcohol anhydrous (99.9%) was purchased from Daejung Chemicals. Bromocresol purple and sodium n-dodecyl sulfate (99%) were purchased from Alfa Aesar and N,N′-methylenebisacrylamide was purchased from Fluka Chemicals. Styrene (≥99%) was purchased from Sigma-Aldrich and purified through a neutral alumina column to remove inhibitors. All other chemicals were used without further purification. Deionized (DI) water (>18.4 MΩ cm) was produced by Milli-Q (Millipore, USA). The synthesized nanoparticles were purified by sequential centrifugations using Supra R22 (Hanil, Korea) and CF-10 (Daihan-Scientific, Korea). Diameters of the synthesized nanoparticles were determined by taking the average of over 100 particle images obtained with a scanning electron microscope (Hitachi S-4800). Absorbance spectra of dyes were obtained on a UV-Vis spectrometer (Agilent 8453); reflectance spectra and CIE (Commission International de l'Eclairage) XYZ colour spaces of HPSs were obtained using an HR2000+ spectrometer (Ocean Optics, USA) equipped with UV-Vis-NIR light source. Photograph images were taken with Nikon D-90.
3
Synthesis of Triethynylbenzene Derivatives
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Materials 1,3,5-Triethynylbenzene (97%), 2,5-dibromothiophene (96%), copper(I) iodide (99%), tetrakis(triphenylphosphine) palladium(0) (99%), potassium carbonate (K 2 CO 3 ) (99%), ammonium oxalate (99%), catalase, sodium nitrate (99%), L-ascorbic acid (99%), triethylamine (99%), 5,5-dimethyl-1-pyrroline N-oxide (97%), 2,2,6,6-tetramethylpiperidine (98%), 5,5-dimethyl-1-pyrroline N-oxide (97%), a-terpinene (90%), mesitylene (98%) and all other solvents were purchased from Sigma Aldrich. Sodium n-dodecylsulfate (99%) was obtained from Alfa Aesar. 4,7-Dibromobenzo[c]-1,2,5-thiadiazole (97%) was purchased from Combi Blocks. Escherichia coli K-12 and Bacillus subtilis were purchased from DSMZ Leibniz-Institute (Braunschweig, Germany). All chemicals and solvents were used as received unless otherwise specified.
4
Synthesis of Multifunctional Polymer Composites
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Polyvinyl alcohol (Polysciences Inc., 88 mol% hydrolyzed) with a molecular weight M w of 205 000 g mol À1 was used as received. 1,3,5-Triethynylbenzene (97%), copper(I) iodide (99%), tetrakis-(triphenylphosphine) palladium(0) (99%), benzofurazan (97%), triethylamine (99%), iron (99%), 2,2,6,6-tetramethylpiperidine (98%) and crystal violet (90%) were purchased from Sigma Aldrich. Glutaraldehyde (GA, 50 wt% aqueous solution) was purchased from Merck KGaA. Sodium n-dodecylsulfate (99%) was obtained from Alfa Aesar. Magnesium sulfate and sodium bicarbonate were purchased from Fisher Chemical. Escherichia coli K-12 and Bacillus subtilis were purchased from DSMZ Leibniz-Institute (Braunschweig, Germany). Demineralized water was used through all the experiments if not specifically mentioned. All chemicals and solvents were used as received unless otherwise specified.
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