Anti progerin

Manufactured by Enzo Life Sciences

Anti-Progerin is a laboratory reagent used to detect and quantify the presence of progerin, a protein associated with premature aging disorders. It functions as a specific antibody that binds to and identifies progerin in biological samples.

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2 protocols using anti progerin

Antibody-Based Protein Analysis Protocol

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Antibodies employed in this study were as follows: anti‐STAT3 phosphorylated on tyrosine 705 (P‐STAT3 Y705), anti‐STAT3 phosphorylated on Serine 727 (P‐STAT3 S727), and anti‐STAT3 from Thermo Fisher Scientific; anti‐γ‐H2AX, rabbit polyclonal (4937; Cell Signaling); anti‐p21, rabbit polyclonal (MA5‐14949; Invitrogen); anti‐Emerin, mouse monoclonal (MONX10804; Monosan); anti‐β‐tubulin; anti‐lamin A/C, goat polyclonal (SC‐6215; Santa Cruz Biotechnology); anti‐Progerin, mouse monoclonal (13A4; Enzo); anti‐GAPDH, mouse monoclonal (MAB374; Millipore); anti‐Ankrd2, mouse monoclonal, clone YAS11 (LS‐Bio); and anti‐collagen VI, monoclonal (Millipore).

Squarzoni S., Schena E., Sabatelli P., Mattioli E., Capanni C., Cenni V., D'Apice M.R., Andrenacci D., Sarli G., Pellegrino V., Festa A., Baruffaldi F., Storci G., Bonafè M., Barboni C., Sanapo M., Zaghini A, & Lattanzi G. (2021). Interleukin‐6 neutralization ameliorates symptoms in prematurely aged mice. Aging Cell, 20(1), e13285.

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Western Blot and Immunofluorescence Antibody Panel

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Antibodies employed for Western blot analysis or immunofluorescence labeling were: Anti-Progerin, mouse monoclonal (ALX-804-662, Enzo Life Sciences); anti-lamin A/C, goat polyclonal (SC-6215, Santa Cruz Biotechnology); anti-prelamin A, goat polyclonal (SC-6214 Santa Cruz Biotechnology); anti-prelamin A, rabbit polyclonal (1188-2, Diatheva), raised against the last 15 aminoacids of the prelamin A sequence including the farnesylated cysteine residue but not the SIM sequence; anti-H3K9me3, mouse monoclonal (AB6001, Abcam) and rabbit polyclonal (07-442, Millipore); anti-monomethyl-H3K9, rabbit polyclonal (Abcam); anti-Histone H3, goat polyclonal (Santa Cruz Biotechnology C-16); anti-BAF, rabbit polyclonal (FL-89, Santa Cruz Biotechnology); anti-LAP2α, rabbit polyclonal (kind gift of Roland Foisner); anti-γH2AX, rabbit polyclonal 4937 Cell Signalling); anti-53BP1, rabbit polyclonal AB2893 Abcam); anti-PARP-1, rabbit polyclonal SC-7150 Santa Cruz Biotechnology); anti-LC3 rabbit polyclonal antibody (NB100-2220, Novus Biological); anti-p62/SQSTM1, guinea pig polyclonal (GP62-C, Progen Biotechnik); anti-actin, goat polyclonal (A1616, Santa Cruz Biotechnology); anti-GAPDH, mouse monoclonal (MAB374, Millipore).

Pellegrini C., Columbaro M., Capanni C., D'Apice M.R., Cavallo C., Murdocca M., Lattanzi G, & Squarzoni S. (2015). All-trans retinoic acid and rapamycin normalize Hutchinson Gilford progeria fibroblast phenotype. Oncotarget, 6(30), 29914-29928.

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