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Human colorectal carcinoma hct116 cells with a heterozygous knockin of idh1 mutant r132h

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Human colorectal carcinoma (HCT116) cells with a heterozygous knockin of IDH1 mutant (R132H). These cells contain a specific genetic modification in the IDH1 gene.

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Lab products found in correlation

Agi 5198, by Cayman Chemical (2 mentions) Bsa palmitate conjugate, by Agilent Technologies (2 mentions)

2 protocols using human colorectal carcinoma hct116 cells with a heterozygous knockin of idh1 mutant r132h

1

Metabolic Profiling of Isocitrate Dehydrogenase Mutant Cells

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All liquid chromatography solvents and additives were obtained from Sigma-Aldrich (St. Louis, MO) and Honeywell Burdick & Jackson (Morris-town, NJ). All cell culture media and reagents were purchased from Thermo Fisher (Mountain View, CA) or Sciencell (Carlsbad, CA). H2O2 was purchased from Sigma-Aldrich (St. Louis, MO). Human colorectal carcinoma (HCT116) cells with a heterozygous knockin of IDH1 mutant (R132H) were obtained from Horizon Discovery (Cambridge, UK). Human immortalized astrocytes were generated as described below. All stable isotopes were purchased from Cambridge Isotope Laboratories (Tewksbury, MO). AGI-5198 was purchased from Cayman Chemical (Ann Arbor, MI). Palmitate-BSA conjugate was purchased from Seahorse Bioscience (Santa Clara, CA).
Gelman S.J., Naser F., Mahieu N.G., McKenzie L.D., Dunn G.P., Chheda M.G, & Patti G.J. (2018). Consumption of NADPH for 2-HG Synthesis Increases Pentose Phosphate Pathway Flux and Sensitizes Cells to Oxidative Stress. Cell reports, 22(2), 512-522.
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2

Metabolic Profiling of Isocitrate Dehydrogenase Mutant Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
All liquid chromatography solvents and additives were obtained from Sigma-Aldrich (St. Louis, MO) and Honeywell Burdick & Jackson (Morris-town, NJ). All cell culture media and reagents were purchased from Thermo Fisher (Mountain View, CA) or Sciencell (Carlsbad, CA). H2O2 was purchased from Sigma-Aldrich (St. Louis, MO). Human colorectal carcinoma (HCT116) cells with a heterozygous knockin of IDH1 mutant (R132H) were obtained from Horizon Discovery (Cambridge, UK). Human immortalized astrocytes were generated as described below. All stable isotopes were purchased from Cambridge Isotope Laboratories (Tewksbury, MO). AGI-5198 was purchased from Cayman Chemical (Ann Arbor, MI). Palmitate-BSA conjugate was purchased from Seahorse Bioscience (Santa Clara, CA).
Gelman S.J., Naser F., Mahieu N.G., McKenzie L.D., Dunn G.P., Chheda M.G, & Patti G.J. (2018). Consumption of NADPH for 2-HG Synthesis Increases Pentose Phosphate Pathway Flux and Sensitizes Cells to Oxidative Stress. Cell reports, 22(2), 512-522.
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