Usp37

Manufactured by Proteintech

Sourced in United States

USP37 is a reference standard material used for the identification and quantification of drugs and other pharmaceutical ingredients. It provides a consistent and reliable reference for analytical methods specified in the United States Pharmacopeia (USP).

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Lab products found in correlation

Protease inhibitor, by Beyotime (1 mentions) 0.45 μm pvdf membrane, by Merck Group (1 mentions) Ripa extraction reagent, by Meilun (1 mentions) Bca kit, by Beyotime (1 mentions) Gapdh, by Proteintech (1 mentions) Snail1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using usp37

Western Blot Analysis of Protein Targets

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Cell pellets were harvested and then lysed using RIPA extraction reagent (Meilun) supplemented with protease inhibitors (Beyotime). The BCA kit (Beyotime) was applied to detect the protein concentration. Afterward, proteins were separated on 10% SDS‐PAGE by electrophoresis and electro‐transferred to 0.45 μm PVDF membrane (Millipore). Antibodies used in this study were ERα (Invitrogen, MA5‐13304), HA (Proteintech, 51064‐2‐AP), Myc (Proteintech, 60003‐2‐Ig), USP37 (Proteintech, 18465‐1‐AP), GST (Proteintech, 66001‐2‐Ig), and GAPDH (Proteintech, 60004‐1‐Ig) antibodies.

Cao J., Wang X., Wang S., Chen Z, & Tang J. (2023). Stabilization of estrogen receptor α by USP37 contributes to the progression of breast cancer. Cancer Science, 114(5), 2041-2052.

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Immunofluorescence Assay for USP37 and Snail1

Cited 1 time

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The IF assay was carried out, as described previously 25 (link). Primary antibodies specific for USP37 (1:100) (Proteintech, Rosemont, IL, USA) and Snail1 (1:100) (Santa Cruz Biotechnology, Dallas, Texas, USA) were applied. Fluorescence images were acquired.

Wu L., Zhao N., Zhou Z., Chen J., Han S., Zhang X., Bao H., Yuan W, & Shu X. (2021). PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1. Theranostics, 11(2), 700-714.

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