Prism version 4 c

For comparisons between groups (control, EAE + IgG2a isotype antibody and EAE + anti-CLEC12A antibody), a two-tailed, unpaired nonparametric t test was used (Mann–Whitney). To determine the significance within a group, a two-tailed, paired nonparametric t test was used (Wilcoxon matched pairs test). The comparison was determined to be significant (*) if the P value was less than or equal to (≤) 0.05, very significant (**) if ≤0.01, and extremely significant (***) if ≤0.001. Statistical values were obtained using Prism version 4 C software (Graphpad Software Inc.).

Data are expressed as mean ± s.d unless otherwise indicated in the figure legends. All relevant data are available from the authors. Analysis was performed using Student’s t-test, single comparison, or analysis of variance (ANOVA) test for multiple comparisons (one-way or two-way ANOVA followed by Tukey’s or Bonferroni’s multiple comparisons test, respectively). All experiments were conducted at least two times. P-values of 0.05 were considered significant and are shown as p<0.05 (*), p<0.01 (**), or p<0.001 (***). Graph generation and statistical analyses were performed using the Prism version 4c software (GraphPad, La Jolla, CA).