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P ser455

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P-Ser455 is an antibody that recognizes the phosphorylated form of the serine 455 residue. This antibody can be used to detect and quantify the phosphorylation of this specific site within target proteins.

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Lab products found in correlation

Pser9 gsk3β, by Cell Signaling Technology (2 mentions) Acrylamide sds page gel, by Bio-Rad (2 mentions) P ser724 ireα, by Cell Signaling Technology (2 mentions) P ireα, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions)

2 protocols using p ser455

1

Adipose Tissue Protein Analysis

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Adipose tissues were homogenized on ice and protein concentrations were determined by Bradford assay as previously described (18) Adipose proteins were fractionated on 4–15% acrylamide SDS-PAGE gels (BioRad, Hercules, CA, USA) and transferred onto nitrocellulose membranes for the western analysis of p-Ser79 ACC, p-Ser455ACL, p-threonine308 kinase Akt/PKB, IR, IRS-1, GLUT4, p-Ser9 GSK3β, p-Ser724 IREα, ACC, ACL, XBP1, p-IREα, IREα, and GAPDH (Cell Signaling, Danvers MA). Proteins signals were quantified by Eagle Eye II Quantitation System (Stratagene, CA, USA).
Liu L., Wang Y., Wang J., Dong Y., Change S., Liu X., Lutfy K., Chen H., Friedman T.C., Jiang M, & Liu Y. (2018). Enhanced Hexose-6-Phosphate Dehydrogenase Expression in Adipose Tissue May Contribute to Diet-Induced Visceral Adiposity. International journal of obesity (2005), 42(12), 1999-2011.
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2

Adipose Tissue Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adipose tissues were homogenized on ice and protein concentrations were determined by Bradford assay as previously described (18) Adipose proteins were fractionated on 4–15% acrylamide SDS-PAGE gels (BioRad, Hercules, CA, USA) and transferred onto nitrocellulose membranes for the western analysis of p-Ser79 ACC, p-Ser455ACL, p-threonine308 kinase Akt/PKB, IR, IRS-1, GLUT4, p-Ser9 GSK3β, p-Ser724 IREα, ACC, ACL, XBP1, p-IREα, IREα, and GAPDH (Cell Signaling, Danvers MA). Proteins signals were quantified by Eagle Eye II Quantitation System (Stratagene, CA, USA).
Liu L., Wang Y., Wang J., Dong Y., Change S., Liu X., Lutfy K., Chen H., Friedman T.C., Jiang M, & Liu Y. (2018). Enhanced Hexose-6-Phosphate Dehydrogenase Expression in Adipose Tissue May Contribute to Diet-Induced Visceral Adiposity. International journal of obesity (2005), 42(12), 1999-2011.
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