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Infinite f200 pro illuminometer

Manufactured by Tecan
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Sourced in Switzerland

The Infinite F200 Pro Illuminometer is a versatile laboratory instrument designed for measuring the light output of various samples. It provides accurate and reliable luminescence detection capabilities to support a wide range of research and testing applications.

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Lab products found in correlation

Coelenterazine, by Santa Cruz Biotechnology (1 mentions) Camp glo kit, by Promega (1 mentions)

2 protocols using infinite f200 pro illuminometer

1

Measuring cAMP Levels in HEK-293 Cells

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To detect cAMP levels, we transfected a Nano-lantern cAMP plasmid (1 µg) and human GPR37 plasmid (1 µg) into HEK-293 cells. BRET activity was measured upon addition of 100 µl/well for a final concentration of 10 µM Coelenterazine (Santacruz), 10 µM drug, and 100 µM forskolin (Enzo, # BML-CN100). Bioluminescent activity was measured using an infinite F200 Pro Illuminometer (Tecan Trading AG, Switzerland). The data displayed indicate the values normalized to basal signal levels.
Bang S., Donnelly C.R., Luo X., Toro-Moreno M., Tao X., Wang Z., Chandra S., Bortsov A.V., Derbyshire E.R, & Ji R.R. (2021). Activation of GPR37 in macrophages confers protection against infection-induced sepsis and pain-like behaviour in mice. Nature Communications, 12, 1704.
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2

Measuring Macrophage cAMP Levels

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Mouse peritoneal macrophage cAMP levels were measured using the cAMP-Glo™ Kit (Promega; V1501), performed according to the manufacturer’s instructions. Macrophage cells treated with forskolin or artesunate. cAMP-mediated bioluminescence was measured using an infinite F200 Pro Illuminometer (Tecan Trading AG, Switzerland).
Bang S., Donnelly C.R., Luo X., Toro-Moreno M., Tao X., Wang Z., Chandra S., Bortsov A.V., Derbyshire E.R, & Ji R.R. (2021). Activation of GPR37 in macrophages confers protection against infection-induced sepsis and pain-like behaviour in mice. Nature Communications, 12, 1704.
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