Estradiol elisa test kit

Manufactured by Neogen

Sourced in United States

The Estradiol ELISA test kit is a laboratory equipment product designed for the quantitative determination of estradiol levels in various biological samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of estradiol in the sample.

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Lab products found in correlation

Vegf elisa test kit, by R&D Systems (2 mentions) Progesterone kit, by Neogen (1 mentions)

3 protocols using estradiol elisa test kit

Quantifying Estradiol and VEGF Levels

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Concentrations of estradiol (E2) and vascular endothelial growth factor (VEGF) in the culture media were measured at day 13 using an estradiol ELISA test kit (Neogen, Lansing, MI) with a detection range of 0–0.2 ng/mL, according to the manufacturer’s instructions. VEGF levels were measured using a VEGF ELISA test kit (R&D Systems, Abingdon, UK), with a detection range of 0–200 ng/mL, according to the manufacturer’s instructions. Interassay and intraassay coefficients of variance in these kits were below 10%.

Nagao S., Baba T., Fujibe Y., Adachi S., Ikeda K., Morishita M., Kuno Y., Honnma H., Endo T., Kiya T, & Saito T. (2019). Pioglitazone suppresses excessive follicular development in murine preantral follicles. Journal of Ovarian Research, 12, 82.

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Measuring E2 and P4 in Cell Culture

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Concentrations of E2 and P4 in culture media were measured at day 13 after culture in low or high FSH condition. E2 levels were measured by an estradiol ELISA test kit (Neogen, MI, USA), with a detective range of 0–2.0 ng/ml, according to the manufacturer’s instructions. P4 levels were measured by a progesterone kit (Neogen), with a detective range of 0–20 ng/ml, according to the manufacturer’s instructions.

Fujibe Y., Baba T., Nagao S., Adachi S., Ikeda K., Morishita M., Kuno Y., Suzuki M., Mizuuchi M., Honnma H., Endo T, & Saito T. (2019). Androgen potentiates the expression of FSH receptor and supports preantral follicle development in mice. Journal of Ovarian Research, 12, 31.

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Quantifying Estradiol and VEGF Levels

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Concentrations of E2 in the culture media were measured at day 10 using an estradiol ELISA test kit (Neogen, Lansing, MI) with a detection range of 0–2.0 ng/mL following the manufacturer’s instructions. VEGF levels in the culture media were measured using a VEGF ELISA test kit (R&D Systems, Abingdon, UK), with a detection range of 0–500 pg/mL, following the manufacturer’s instructions. Interassay and intraassay coefficients of variance in these kits were below 10%.

Kasuga-Yamashita F., Baba T., Nagao S., Fujibe Y., Morishita M., Kuno Y., Mariya T., Honnma H., Endo T., Kiya T, & Saito T. (2022). Letrozole increases preantral follicle growth and decreases estradiol production without impairing follicle survival. Journal of Ovarian Research, 15, 136.

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