Immunohistochemical analyses were performed by the Histology facility at the CRUK Beatson Institute using standard protocols on Bond Rx or Dako autostainers. Anti‐Ki‐67 (clone SP6; 1 : 100) was purchased from Abcam, and anti‐caspase 3 (clone Asp‐175; 1 : 500) was purchased from Cell Signaling. Quantitative analysis of positive staining was performed by counting cells in at least three high‐power fields of view (×40) per tumor by two independent researchers who were blinded to the sample group. Images were captured on an Axio Imager A2 Bio upright microscope (Zeiss, Cambridge, UK) using zenpro 2012 software (Zeiss).
Axio imager a2 bio upright microscope
Manufactured by Zeiss
Sourced in United Kingdom
The Axio Imager A2 Bio is an upright microscope designed for routine biological applications. It features advanced optics and a sturdy ergonomic design to provide high-quality imaging performance.
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2 protocols using axio imager a2 bio upright microscope
1
Immunohistochemical Analysis of Tumor Samples
2
Quantitative Immunohistochemical Analysis
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Immunohistochemical analyses were performed by the Histology facility at the CRUK Beatson Institute using standard protocols on Bond Rx or Dako autostainers. Anti-Ki67 (clone SP6; 1:100) was purchased from Abcam and anti-Caspase 3 (clone Asp-175; 1:500) was purchased from Cell Signaling. Quantitative analysis of positive staining was performed by counting cells in at least 3 high-power fields of view (x40) per tumor by two independent researchers who were blinded to the sample group. Images were captured on an Axio Imager A2 Bio upright microscope (Zeiss) using ZenPro 2012 software (Zeiss).
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