Fluoview fv10 asw 1.7c software

To determine if the ratio of interneurons to principal cells was changing in a given hippocampal region, we divide the density of PV neurons (in the pyramidal layer of the CA1 or the granule layer of the DG) by the density of Cresyl Violet stained cells in the same region. We did this for a total sample area of 6000 μm² in each cell layer. The resulting number is reported as a “inhibitory cell ratio”. We further subdivided the hippocampus into dorsal (bregma −1.88 to −4.16) and ventral (bregma −4.52 to −6.04) subregions to help determine if there were regional differences in PV cell density using this inhibitory cell ratio. All statistics using the general linear model were done using IBM SPSS statistics software and estimation statistics and figures were made using R (Stull, 1994 ; R Computing, 2018 ). A 95% confidence interval was used as the statistical threshold in this study, and all post-hoc tests were performed using a Tukey test.
Representative fluorescent images were taken using and Olympus BX61WI confocal laser scanning microscope and Olympus FluoView FV10-ASW 1.7c software (Olympus Corporation, Center Valley, PA, USA).