The most appropriate condition was determined using a combination of different concentrations of CSE stimulation at different exposure times of 3, 6, 12, 24, and 48 hours. Basically, 30% CSE was the maximal dilution without excessive cell mortality after 24 hours exposure. For treatment with U-75302, RAW264.7 cells were seeded overnight and treated with 20% CSE with 10 nmol/mL U-75302 (Enzo, Telluride, CO, USA) for 24 hours. Supernatant was then harvested and total cellular RNA and protein were extracted.
U75302
Manufactured by Enzo Life Sciences
Sourced in United States
The U75302 is a laboratory equipment product from Enzo Life Sciences. It is a piece of equipment used for scientific research and analysis, but a detailed description of its core function cannot be provided in a concise, unbiased, and factual manner without potential for extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Ly255283, by Cayman Chemical (3 mentions)
Baicalein, by Enzo Life Sciences (2 mentions)
Recombinant mouse il 1β, by R&D Systems (1 mentions)
Mcc950, by Thermo Fisher Scientific (1 mentions)
Ac yvad cmk, by Enzo Life Sciences (1 mentions)
Lps escherichia coli serotype o55 b5, by Merck Group (1 mentions)
4 protocols using u75302
1
Optimizing CSE Exposure for Cell Viability
2
Investigating NLRP3 Inflammasome Inhibition
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Recombinant mouse IL-1β was obtained from R&D Systems (Minneapolis, MN, USA). MCC950 (NLRP3 inhibitor, cat no. inh-mcc) was obtained from Invitrogen (San Diego, CA, USA). Lipopolysaccharide (LPS, Escherichia coli serotype O55:B5), dexamethasone (cat no. D4902), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MK886 (5-LOX inhibitor, cat no. 475889) was obtained from Calbiochem (La Jolla, CA, USA). Ac-YVAD-cmk (caspase-1 inhibitor, cat no. ALX-260-028), U75302 (BLT1 antagonist, BML-RA102-0100) and baicalein (12-LOX inhibitor, BML-EI106-0050) were obtained from Enzo Life Sciences (Farmingdale, NY, USA). LY255283 (BLT2 antagonist, cat no. 70715) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Antibodies against NLRP3 and caspase-1 were purchased from AdipoGen Life Sciences (San Diego, CA, USA).
3
Inhibition of Inflammatory Pathways
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LPS (Escherichia coli serotype O55:B5) and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St Louis, MO). The BLT1 antagonist U75302 and the 12-LO inhibitor Baicalein were obtained from Enzo Life Sciences (Farmingdale, NY). The BLT2 antagonist LY255283 was obtained from Cayman Chemical (Ann Arbor, MI). The 5-LO inhibitor MK886 and the NF-κB inhibitor Bay11-7082 were obtained from Calbiochem (La Jolla, CA).
4
Dimethyl Sulfoxide and Receptor Antagonists
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Dimethyl sulfoxide (DMSO) was obtained from Sigma-Aldrich (USA). U75302 was obtained from Enzo Life Sciences (USA). LY255283 was obtained from Cayman Chemical (USA).
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