Dr. Dong at Tulane University provided the pMD-VSV-G and delta 8.2 plasmids. Bim and non-target control (NTC) shRNA lentiviral vectors were purchased from Sigma-Aldrich. Red fluorescent protein (RFP) and Mcl-1 cDNA constructs were purchased from Thermo Fisher Scientific Biosciences Lafayette, CO, USA). Lentivirus production and transduction were carried out using Lipofectamine and Plus reagents Life Technologies), as previously described [12 (link),27 (link)].
Ntc shrna lentiviral vectors
Manufactured by Merck Group
NTC) shRNA lentiviral vectors are a type of lab equipment used for genetic manipulation and gene expression studies. They contain a non-targeting control (NTC) short hairpin RNA (shRNA) sequence packaged in a lentiviral vector. These vectors can be used to transduce cells and express the NTC shRNA, which serves as a control for experiments involving RNA interference (RNAi) techniques.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ntc shrna lentiviral vectors
1
Lentiviral Vector Production and Transduction
2
Lentiviral shRNA Transduction Protocol
3
Lentiviral Transduction of Bim and Mcl-1 Constructs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pMD‐VSV‐G and delta 8.2 plasmids were gifts from Dr. Dong at Tulane University. Bim and non‐target control (NTC) shRNA lentiviral vectors were purchased from Sigma‐Aldrich. Precision LentiORF Mcl‐1 and RFP (red fluorescent protein) lentivirus vector were purchased from Dharmacon (Lafayette, CO, USA). Lentivirus production and transduction were carried out as previously described.46 Briefly, TLA‐HEK293T cells were transfected with pMD‐VSV‐G, delta 8.2, and lentiviral shRNA or Precision LentiORF constructs using Lipofectamine and Plus reagents (Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions. Virus containing culture medium was harvested 48 hours post transfection. Cells were transduced overnight using 1 mL of virus supernatant and 4 μg of polybrene and then cultured for an additional 48 hours prior to selection with puromycin or blasticidin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!