Plb lysis buffer

Manufactured by Promega

PLB lysis buffer is a general-purpose lysis buffer used for the extraction and purification of nucleic acids from cells and tissues. It is designed to efficiently lyse a wide range of sample types, including mammalian, bacterial, and plant cells. The buffer composition facilitates the release of nucleic acids while maintaining their integrity.

Automatically generated - may contain errors

Lab products found in correlation

Dual glo luciferase assay, by Promega (1 mentions)

Spelling variants of this product

Passive lysis buffer (2573 citations) Lysis buffer (395 citations) PLB buffer (9 citations) 1X Passive Lysis Buffer (PLB, (3 citations)

2 protocols using plb lysis buffer

Luciferase Reporter Assay in C2C12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

pLuc empty vector, pLuc-UTR vector, pLuc-intr.-UTR vector, pLuc-EMCV-IRES vector and pLuc-intr.-UTR-D-Spl. vector were transfected in C₂C₁₂ cells as follows: 1 μL of lipofectamine 2000 was added to 100 μL of Optimem together with 500 ng of DNA, mixed and pipetted onto 50,000 pre-seeded cells in a 12 well plate. Medium was replaced 4 hr later and cells harvested for subsequent analyses after 48 hr.
Cells were harvested with PLB lysis buffer (Promega) and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega) according to the manufacturer’s protocol. A part of cell lysate was kept for RNA extraction and checked by RT-PCR with FLuc-pGL3control_FW and RLuc-pRLTK_RV oligonucleotides.

Legnini I., Di Timoteo G., Rossi F., Morlando M., Briganti F., Sthandier O., Fatica A., Santini T., Andronache A., Wade M., Laneve P., Rajewsky N, & Bozzoni I. (2017). Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis. Molecular Cell, 66(1), 22-37.e9.

+ Open protocol

+ Expand

Dual-Luciferase Assay for Circadian Rhythms

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells were co-transfected with a combination of five plasmids driving expression of Per2 or Per2-FLuc, NanoLuc (PE-box-NLuc), Renilla Luciferase (PCMV-RLuc), Bmal1, and Clock (Bmal1 and Clock have no luciferase tags). PCMV is the standard CMV promoter used in the Dual-Luciferase assay, and PE-box is a synthetic promoter that contains 3 canonical E-box motifs (CACGTG) in tandem. We modified the standard transient transfection Dual-Luciferase assay [72 (link)] to use NanoLuc instead of FLuc because the presence of FLuc on PER2::FLuc interferes with the standard Dual-Luciferase assay. Coelenterazine substrate was used to visualize RLuc with the Dual-luciferase kit (E1910). Furimazine substrate was used for NLuc with the Nano-Glo kit (N-1610). Transfected cells were extracted with 1X PLB lysis buffer (Promega E-194A) one day (24 h) after transfection, and measurements for the various luciferases and assessment of potential cross-reactivity were conducted using the same amount of cell extract. The forward primer for mPer2Luc () was designed to be upstream of the deleted region. The reverse primer () was designed to be at the 5-prime end of firefly luciferase (FLuc).

Ralph M.R., Shi S.Q., Johnson C.H., Houdek P., Shrestha T.C., Crosby P., O’Neill J.S., Sládek M., Stinchcombe A.R, & Sumová A. (2021). Targeted modification of the Per2 clock gene alters circadian function in mPer2luciferase (mPer2Luc) mice. PLoS Computational Biology, 17(5), e1008987.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!