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Mts50 z8

Manufactured by Thorlabs

The MTS50-Z8 is a motorized translation stage from Thorlabs. It provides 50 mm of travel along the Z-axis. The stage is driven by a stepper motor and can be controlled using the TDC001 T-Cube DC Servo Motor Controller or other compatible control systems.

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3 protocols using mts50 z8

1

Automated Calibration Tool for Optical Measurements

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A three-dimensional rendering of the automated calibration tool is shown in Fig. 1. The tool uses a motorized stage (MTS50-Z8, ThorLabs) connected to a customized fixture holding six unique calibration components. These components are a background fixture, a white reflectance standard, a flat-fielding standard, a wavelength calibration lamp fixture, and two optically scattering phantoms. Each of these components will be described in detail. To use the fixture, the fiber-optic probe is inserted into the top of the fixture and pushed down until the probe hits a stopper. The stopper holds the probe 0.2 mm above the fixtures. This prevents the probe from touching any of the substrates directly and damaging them. A computer controls the movement of the stage and acquisition of measurements from each calibration fixture. The stage automatically moves each calibration standard under the probe for measurements to be acquired.
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2

Photoacoustic Imaging of Tissue Samples

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Samples were placed on a 2D scanning system, consisting of 2 linear motorized stages (MTS50-Z8, Thorlabs Inc., Newton, NJ). The excitation beam was amplitude-modulated at a single frequency typically between 100 KHz and 1 MHz. All PA images shown in this manuscript were taken at 1 MHz laser modulation. Two-dimensional images were obtained by raster scanning the linear stages and recording the modulated probe signal, which was proportional to the amplitude of the PA wave produced at that location, using a lock-in amplifier (UHFLI, Zurich Instruments, Zurich, Switzerland) that provided 120 dB of dynamic reserve. Typical integration time used was 10 ms. Images were acquired using a 50-ms dwell time at each location.
When imaging tissue, the total optical intensity focused on the sample was adjusted to avoid photothermal damage. Laser spot size on the tissue surface was adjusted to be about 10 μm in diameter. The minimum probe beam power that gives a detectable signal at the PD was selected. For a tissue sample, this criterion resulted in a higher incident intensity for the probe beam because the reflection was typically less than 1%.
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3

Optical Monitoring of Redox-Active Hydrogel

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For electrochemical measurement and deposition, a CHI6273C electrochemical analyzer was used. UV-Vis measurements were performed on a Thermo Scientific Evolution 60 spectrophotometer. Mass Spectrometry measurements were obtained using a Waters SQ Detector 2 single quadrupole mass spectrometer, combined with a Waters Alliance® e2695 HPLC system equipped with a dual absorbance 2489 UV/Vis detector. The fluorescence images were captured using an inverted microscope (Olympus BX60).
To monitor the redox state change of PYO-PEG hydrogel optically, a custom motorized optical absorbance meter was designed and constructed. As shown in Figure S7, it consists of a stepper motor positioning platform (Thorlabs MTS50-Z8) which moves a co-located 750nm LED (Thorlabs LED750L), its driver (Thorlabs LEDMT1E), a 25μm pinhole (Thorlabs P25HK), a collimating lens (Thorlabs AL1225M), and a high gain amplified Si photodetector (Thorlabs PDA100A2) on the opposing side.
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