Sections were washed with phosphate buffer saline (PBS) before being incubated in a blocking solution (5% donkey serum in 0.4% Triton X-100 PBS). Antibodies to NR4A2 (1:200, Abcam, ab41917 RRID:AB_776887), SATB2 (1:200, Abcam, ab51502, RRID:AB_882455), TBR1 (1:500, Abcam, ab31940, RRID: AB_2200219), NeuN (1:500, EMD Millipore, ABN78, RRID: AB_10807945), SOX5 (1:200, Abcam, ab94396, RRID: AB_10859923), CTIP2 (1:200, Abcam, ab18465, RRID: AB_2064130), Parvalbumin (1:1,000, Sigma, P3088, RRID: AB_477329) were incubated overnight at 4 °C in PBS with 2% donkey serum. After washing three times in phosphate buffer saline tween, sections were incubated with species-specific secondary antibodies for 2 h at room temperature. Secondary antibodies were obtained from Jackson ImmunoResearch. For single immunofluorescence labeling, either species-antibodies labeled with Alexa 488 or Cy3 were used. For double labeling, Alexa 488 was combined with Cy3 or Cy5. Sections were washed again three times and then mounted with Vectashield Mounting Medium for Fluorescence (Vector Laboratories Inc.).
Ab41917
Manufactured by Abcam
Sourced in United States
Ab41917 is a lab equipment product. It is a device used in scientific research and analysis. The core function of this product is to facilitate specific laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab51502, by Abcam (1 mentions)
Ab94396, by Abcam (1 mentions)
Ab18465, by Abcam (1 mentions)
Vectashield mounting medium for fluorescence, by Vector Laboratories (1 mentions)
Abn78, by Abcam (1 mentions)
P3088, by Merck Group (1 mentions)
Ab8049, by Abcam (1 mentions)
Ab7751, by Abcam (1 mentions)
Ab290, by Abcam (1 mentions)
Ab6211, by Abcam (1 mentions)
Fitc conjugated anti mouse igg, by Merck Group (1 mentions)
D9542, by Merck Group (1 mentions)
Normal goat serum (ngs), by Merck Group (1 mentions)
N5413, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
2 protocols using ab41917
1
Immunofluorescence Labeling of Neuronal Markers
2
Immunofluorescence Assessment of Neural Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Evaluation of the specific protein profiles of the differentiated cells carried out by immunofluorescence. The cells of all the samples were fixed in 4% paraformaldehyde and rinsed with PBS. Then, they permeabilized by 0.3% Triton X-100 and incubated with blocking buffer (10% normal goat serum; NGS, Sigma, G9023). The cells subsequently incubated with primary antibodies, including rabbit polyclonal nestin (Sigma, N5413), mouse anti-synaptophysin monoclonal (Abcam, ab8049), mouse anti-β-III tubulin monoclonal (Abcam, ab7751), rabbit anti-tyrosine hydroxylase–neuronal marker (Abcam, ab6211), rabbit polyclonal antibody to GFP (Abcam, Cambridge, USA, ab290), and mouse monoclonal anti-Nurr1 antibody (Abcam, ab41917), each at 1:1,000 dilutions. FITC-conjugated anti-mouse IgG (Sigma, F9137) and Cy5.29-conjugated anti-rabbit IgG (Abcam, ab6564) each at 1:1,000 dilutions applied as secondary antibodies. DAPI (4′,6-Diamidino-2-phenylindole, Sigma, D9542) utilized to counterstain the nuclei.
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