Immunolabeling of fixed guts were carried out as before42 ,50 (link). Primary antibodies used in this study were rat anti-mCherry (1:300), rabbit anti-p62 (1:2,000)31 (link), chicken anti-GFP (1:1,500, Invitrogen, A-10262), mouse anti-Delta (1:100, DSHB, C594.9B), rabbit anti-phospho-histone H3 (1:300, Millipore, 06–570), mouse anti-Prospero (1:100, DSHB, MR1A), rabbit anti-Pdm1 (1:50, gift of Fernando Jimenez Diaz-Benjumea), rabbit anti-ubiquitin (1:500, DAKO), mouse anti-H2Av (1:50, DSHB, UNC93-5.2.1), rabbit anti-Cdc2 (1:200, Santa Cruz Biotechnology, sc-53), rabbit anti-Cleaved Caspase 3 (1:50, Cell Signaling, 9661), rat anti-Atg8a (1:300)51 (link) and secondary antibodies Alexa Fluor 488 anti-chicken A11039, Alexa Fluor 568 anti-rabbit A11011, Alexa Fluor 568 anti-mouse A11004 (all 1:1,500; Invitrogen). All stainings were repeated at least once, with similar results.
Rabbit anti cdc2
Manufactured by Santa Cruz Biotechnology
Sourced in Germany
Rabbit anti-Cdc2 is a primary antibody that targets the Cdc2 protein, also known as Cyclin-Dependent Kinase 1 (CDK1). Cdc2 is a key regulator of cell cycle progression and is involved in the control of the G1/S and G2/M transitions.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti phospho histone h3, by Merck Group (1 mentions)
Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Chicken anti gfp, by Thermo Fisher Scientific (1 mentions)
Rabbit anti ubiquitin, by Agilent Technologies (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Rabbit anti ho1, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rabbit anti cdc2
1
Immunolabeling of Fixed Gut Tissues
2
Protein Expression Analysis in Keratinocytes
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Total cell lysates were prepared from HHFKs cultivated in the indicated culture conditions. After separating 5–10 µg protein by using NuPAGE® 4–12% Bis-Tris gels in the XCell SureLockTM electrophoresis cell system, proteins were transferred onto a polyvinylidene fluoride membrane and were blocked with 2% bovine serum albumin in 1 x Tris-buffered saline + 0.05% Tween 20 for 4–16 h. The following primary antibodies were used: mouse anti-β-actin (Sigma-Aldrich®; Munich, Germany), rabbit anti-CHAC1, rabbit anti-CDC2, rabbit anti-HJURP, and rabbit anti-HO1 (all from Santa Cruz Biotechnology; Heidelberg, Germany). Proteins were visualized indirectly using horseradish peroxidase-conjugated secondary antibodies (anti-mouse and anti-rabbit; Jackson ImmunoResearch; PA, USA) and enhanced chemiluminescence immunodetection. Western Blots were quantified by densitometry (ImageJ software). Total protein levels were normalized to the levels of a housekeeping gene. The protein levels after IC treatment are shown in relation to levels detected in HHFKs cultured only in MGM. CDK1 protein levels after treatment with L-cystine were related to levels detected in IC-cultured HHFKs.
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