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Bbl thioglycolate medium

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BBL thioglycolate medium is a microbiology culture medium used for the cultivation and isolation of anaerobic and aerobic bacteria. It contains thioglycollate, which acts as a reducing agent to create an anaerobic environment, and provides nutrients to support the growth of a variety of microorganisms.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi cell culture medium, by Thermo Fisher Scientific (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Lipopolysaccharides (lps), by Enzo Life Sciences (1 mentions)

Spelling variants of this product

Thioglycolate (18 citations) 3% thioglycolate medium (9 citations)

3 protocols using bbl thioglycolate medium

1

Cultivation and Isolation of Primary Macrophages

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Unless otherwise indicated, all cells were cultured at 37 °C in 95% air and 5% CO2. Peritoneal macrophages were elicited by intraperitoneal injection of 1 mL BBL thioglycolate medium, brewer modified (4%; BD), and then they were recovered 4d later by peritoneal lavage with 20 mL PBS. The peritoneal macrophages were cultured in RPMI cell culture medium (Gibco) containing 10%FBS (Gibco), 1% penicillin and streptomycin. Primary BMDMs were grown for 6 days in DMEM supplemented with 10% FBS (Gibco), 30% medium conditioned by L929 mouse fibroblasts, and 1% penicillin and streptomycin. BMDMs in antibiotic-free medium were seeded onto 6-well plates at a density of 2×10^6 cells per well, followed by incubation overnight. L929 cells were cultured in RPMI-1640 medium supplemented with 10% FBS. 2 mM glutamine, 1% penicillin and streptomycin. HEK293T cells were cultured in DMEM supplemented with 10% FBS, 2mM glutamine and 1% penicillin and streptomycin.
Guan C., Huang X., Yue J., Xiang H., Shaheen S., Jiang Z., Tao Y., Tu J., Liu Z., Yao Y., Yang W., Hou Z., Liu J., Yang X.D., Zou Q., Su B., Liu Z., Ni J., Cheng J, & Wu X. (2021). SIRT3-mediated deacetylation of NLRC4 promotes inflammasome activation. Theranostics, 11(8), 3981-3995.
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2

Quantifying Macrophage Efferocytosis of Apoptotic Cells

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Phagocytosis by inflammatory macrophages was evaluated by injecting 1×107 apoptotic thymocytes or PBS alone (control without phagocytosis) 72 hours after the initial peritoneal injection of thioglycolate (3%; BBL Thioglycolate Medium, brewer modified, BD Biosciences). Three hours after injection, 1 mL PBS was injected into the peritoneum and collected. Peritoneal lavage fluid was centrifuged. Supernatants were harvested and frozen before VEGFA quantification. To evaluate the amount of efferocytosis by flow cytometry, cells were collected and stained with the following antibodies: Alexa 488–conjugated anti-CD11b and V450-conjugated anti-Ly6G (BD Biosciences). The amount of efferocytosis by monocytes/macrophages was expressed as arbitrary fluorescence unit.
Howangyin K.Y., Zlatanova I., Pinto C., Ngkelo A., Cochain C., Rouanet M., Vilar J., Lemitre M., Stockmann C., Fleischmann B.K., Mallat Z, & Silvestre J.S. (2016). Myeloid-Epithelial-Reproductive Receptor Tyrosine Kinase and Milk Fat Globule Epidermal Growth Factor 8 Coordinately Improve Remodeling After Myocardial Infarction via Local Delivery of Vascular Endothelial Growth Factor. Circulation, 133(9), 826-839.
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3

Isolation and Culture of Mouse Peritoneal Macrophages

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Thioglycolate-elicited macrophages were recovered 4 d after i.p.
injection of 2 mL BBL thioglycolate medium, brewer modified (4%; BD
Biosciences) by peritoneal lavage with 5 mL phosphate buffered saline (PBS).
The peritoneal macrophages were cultured in DMEM cell culture medium [DMEM
containing 10% FBS (Gemini Bio Products), 1% penicillin and
streptomycin (Life Technologies)] at 37 °C and 95%
air/5% CO2. Cells were seeded onto 96-well plates at 1
× 105 cells per well and treated with 5, 6,
or the candidate analogues (dissolved in DMSO, and final assay DMSO
concentrations (≤0.5%) were kept constant in all
experiments) and ultra-pure LPS (dissolved in H2O, Enzo Life
Sciences) for 4 h and assessed alongside DMSO treated controls.40 (link) Mouse TNF-α in the
supernatants was measured by ELISA kits according to the
manufacturer’s instructions (eBioscience and PBL Assay Science). All
data is mean of triplicates and SD is within +10% of mean. Mouse
cells were from wild type C57BL/6J mice.
Morin M.D., Wang Y., Jones B.T., Su L., Surakattula M.M., Berger M., Huang H., Beutler E.K., Zhang H., Beutler B, & Boger D.L. (2016). Discovery and Structure–Activity Relationships of the Neoseptins: A New Class of Toll-like Receptor-4 (TLR4) Agonists. Journal of medicinal chemistry, 59(10), 4812-4830.
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