Toyopearl hw 65s

Rigid 20–40 μm beads made from hydroxylated methacrylic polymer (Toyopearl HW-65S, Tosoh Bioscience) are used to demonstrate the formation of monodispersed bead-containing droplets at limiting dilution. Beads are suspended in 68% OptiPrep DensityGradient Medium (Sigma-Aldrich), and co-flowed with 2% agarose (Ultra-low Gelling Temperature Agarose, Type IX-A, Sigma-Aldrich) in 1× PBS. The device, identical to one previously described,1 (link) is fabricated at a height of 100 μm. Flow rates are 2000 μl h^–1 bead-containing solution, 2000 μl h^–1 agarose, and 40 000 μl h^–1 2% ionic Krytox, prepared as previously described.18 (link)

Pediococcus pentosaceus MYU 759 was propagated at 37°C for 24 hr using one liter of MRS broth. An equal volume of 99.5% cold ethanol (Kanto Chemical Co., Inc.) was added to
the supernatant after centrifugation (1,500 × g, 15 min, 4°C), and the mixture was allowed to stand at 4°C overnight, followed by centrifugation (12,200 × g, 30 min, 4°C) to obtain a
precipitate. After treatment with 10% trichloroacetic acid, the sugar fraction was obtained by ethanol precipitation. Next, it was applied to DEAE-TOYOPEAL 650 M (Tosoh Bioscience, King of
Prussia, PA, USA) followed by TOYOPEARL HW-65S (Tosoh Bioscience). The fractions with a high sugar content were dialyzed against distilled water at 4°C for 2 days and then lyophilized. The
purified EPSs were dissolved with assay buffer (0.125, 0.25, and 0.5 mg/ml), and the antioxidant capacity was measured by the ORAC and HORAC methods. The data were indicated as the Trolox
equivalent antioxidant capacity (TEAC; μM TE) and gallic acid equivalent antioxidant capacity (GAEAC; μM GAE).