Degummed B. mori silkworm silk fiber was supplied by BJ Silk Co. Ltd (Jinju-si, Korea). Calcium chloride dihydrate (71.0%–77.5%) and ethanol (94.5%) were purchased from Samchun Chemical (Pyeongtaek, Korea). Dialysis cellulose membranes were purchased from Sigma-Aldrich Co. (cutoff 12,000–13,000 Da) and Membrane Filtration Products Inc. (Cellu-Sep 5030-43, cutoff 3,500 Da) and presoaked in distilled water for 10 minutes before use. Polyethylene glycol was purchased from KPX Green Chemical Co. Ltd (Seoul, Korea). Phosphate buffer solution (1.0 M, pH 7.4), protease from Streptomyces griseus, rhodamine B (Rho), and fluorescein (Flu) were purchased from Sigma-Aldrich Co. All the chemical agents were used without further purification.
Phosphate buffer solution 1.0 m
Manufactured by Merck Group
Sourced in Germany
Phosphate buffer solution (1.0 M) is a laboratory reagent used to maintain a specific pH in chemical and biological experiments. It is a mixture of sodium phosphate monobasic and sodium phosphate dibasic solutions at a concentration of 1.0 molar.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using phosphate buffer solution 1.0 m
1
Silk Fiber Regeneration and Labeling
2
Synthesis of Functional Hydrogels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Reagents: N,N’-methylene bisacrylamide (BIS) (CAS 110-26-9), Acrylamide (Aam) (CAS 79-06-1), N-tert-butylacrylamide (TBAm) (CAS 107-58-4), 2-hydroxyethyl methacrylate(HEMA) (CAS 868-77-9), ammonium persulfate (APS) (CAS 7727-54-0), N,N,N’,N’-tetramethylethylenediamine (TEMED) (CAS 110-18-9, sodium dodecyl sulfate (SDS) (CAS 151-21-3), phosphate buffer solution 1.0 M were from Sigma-Aldrich (Darmstadt, Germany) and used without any further purification. All other chemicals were of analytical reagent grade. The solvent was Milli-Q water.
The bovine serum albumin (CAS 9048-46-8) and trypsin (CAS 9002-07-7) were from Sigma-Aldrich (Darmstadt, Germany).
The bovine serum albumin (CAS 9048-46-8) and trypsin (CAS 9002-07-7) were from Sigma-Aldrich (Darmstadt, Germany).
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