Cells were plated in triplicate in a six-well plate at a density of 1 ×105 cells/well and incubated for 24 h. Then, cells were treated with different concentrations of aspirin (0 µM, 100 µM, and 1,000 µM) and/or CDDP (20 µM). After a 48-h incubation at 37 °C, cells were harvested, trypsinized and washed with cold phosphate-buffered saline (PBS). Subsequently, cells were stained with APC Annexin V and propidium iodide (PI) (BioLegend, Inc., San Diego, CA, USA). The stained cells were immediately analyzed using an LSR flow cytometer (Beckman Coulter, Inc., 250 S. Kraemer Boulevard, Brea, CA, USA), and the data were analyzed using Summit 6.2 software (Beckman Coulter, Inc., 250 S. Kraemer Boulevard, Brea, CA, USA).
Lsr flow cytometer
Manufactured by Beckman Coulter
Sourced in United States
The LSR flow cytometer is a versatile and advanced instrument designed for high-performance cell analysis. It utilizes laser light scattering and fluorescence detection to provide precise measurements of various cellular parameters, including size, granularity, and the expression of specific surface markers or intracellular molecules. The LSR flow cytometer is a reliable and efficient tool for a wide range of applications in biological research, clinical diagnostics, and cell-based assays.
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Lab products found in correlation
2 protocols using lsr flow cytometer
1
Apoptosis Induction by Aspirin and CDDP
2
Multicolor Flow Cytometry Analysis of PBMC
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Isolated PBMC from patients and controls were stained with monoclonal antibodies reactive against the cell surface markers CD19, CD27, and CD43 purchased from BD Biosciences (East Rutherford, NJ) and CD24 and CD38 from BioLegend (San Diego, CA). Multicolor flow cytometry was performed for all samples on a Beckman Coulter (Sharon Hill, PA) LSR flow cytometer using standardized voltage and compensation settings. Gating was determined using healthy control samples and was consistent throughout the study. Data analysis was performed using Tree Star (Ashland, OR) FlowJo version X.0.6 software.
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