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Cd80 b7 1 monoclonal antibody

Manufactured by Thermo Fisher Scientific
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CD80/B7–1 Monoclonal Antibody is a laboratory reagent used for the identification and characterization of the CD80 (B7-1) protein. CD80 is a co-stimulatory molecule expressed on antigen-presenting cells that plays a key role in T-cell activation and immune response regulation.

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Lab products found in correlation

Macrophage base medium xf, by PromoCell (2 mentions) M1 macrophage generation medium xf, by PromoCell (2 mentions) Alexa fluor 488 dye, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Ecori, by Takara Bio (1 mentions) Cell counting kit, by Beyotime (1 mentions) Tlr5 antibody, by Proteintech (1 mentions) P erk1 2 antibody, by Abcam (1 mentions) Bamhi, by Takara Bio (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Red blood cell lysing buffer, by Solarbio (1 mentions) Porcine peripheral blood lymphocyte separation kit, by Solarbio (1 mentions) Minimacs starting kit, by Miltenyi Biotec (1 mentions) Mouse igg1 kappa isotype control, by Thermo Fisher Scientific (1 mentions) Goat anti pig igm, by Bio-Rad (1 mentions) Anti fitc microbeads, by Miltenyi Biotec (1 mentions)

4 protocols using cd80 b7 1 monoclonal antibody

1

Differentiation of PBMCs into M1 Macrophages

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Frozen PBMCs from individual donors without cancer were obtained from UW-Carbone Cancer Center Biobank. PBMCs were differentiated into M0 and M1 macrophages using Macrophage Base Medium XF (Item No. C-28057) and M1-Macrophage Generation Medium XF (Item No. C-28055) from Promo Cell according to manufacturer’s instructions in NUNC 6-well cell culture plates (Item No. 140675). Macrophage CD80 was detected using CD80/B7–1 Monoclonal Antibody (Item No. 66406–1-IG) from Thermo Fisher conjugated to Alexa Fluor 488 Dye (immunofluorescence 1:100).
Hu Y., Manasrah B.K., McGregor S.M., Lera R.F., Norman R.X., Tucker J.B., Scribano C.M., Yan R.E., Humayun M., Wisinski K.B., Tevaarwerk A.J., O’Regan R.M., Wilke L.G., Weaver B.A., Beebe D.J., Jin N, & Burkard M.E. (2021). Paclitaxel induces micronucleation and activates pro-inflammatory cGAS-STING signaling in triple-negative breast cancer. Molecular cancer therapeutics.
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2

Differentiation of Frozen PBMCs into M0 and M1 Macrophages

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Frozen PBMCs from individual donors without cancer were obtained from UW-Carbone Cancer Center Biobank. PBMCs were differentiated into M0 and M1 macrophages using Macrophage Base Medium XF (Item No. C-28057) and M1-Macrophage Generation Medium XF (Item No. C-28055) from Promo Cell according to manufacturer’s instructions. Macrophage CD80 was detected using CD80/B7–1 Monoclonal Antibody (Item No. 66406–1-IG) from Thermo Fisher conjugated to Alexa Fluor 488 Dye (immunofluorescence 1:100).
Hu Y., Manasrah B.K., McGregor S.M., Lera R.F., Norman R.X., Tucker J.B., Scribano C.M., Yan R.E., Humayun M., Wisinski K.B., Tevaarwerk A.J., O’Regan R.M., Wilke L.G., Weaver B.A., Beebe D.J., Jin N, & Burkard M.E. (2021). Paclitaxel induces micronucleation and activates pro-inflammatory cGAS-STING signaling in triple-negative breast cancer. Molecular cancer therapeutics.
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3

Flagellin-mediated immune response study

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RNAiso plus 9109 Q (TaKaRa Tokyo, Japan); Hifair® III 1st Strand cDNA Synthesis Kit (gDNA digester plus)11139 ES 10 (YEASEN, Shanghai, China); Hieff UNICON® Universal Blue Qpcr SYBR Green Master Mix 11184 ES 03 (YEASEN); Recombinant flagellin protein derivative CBLB502 (InvivoGen, Toulouse, France); TIRF-specific antagonist Pepinh-TRIFTFA (MCE, Sichuan, China); EMEM culture medium (ATCC, Manassas, VA, USA); DMSO (Sigma-Aldrich, St. Louis, MO, USA); Fetal bovine serum (Gibco, Waltham, MA, USA); Plasmid mini extraction kit (Omega, Norcross, GA, USA); Plasmid maxi extraction kit (Omega, Norcross, GA, USA); EcoRI (TaKaRa, Japan); BamHI (TaKaRa); RNAisoplus (TaKaRa); TLR5 antibody (rabbit source; Proteintech, Rosemont, IL, USA); TRIF antibody (rabbit source, Affinity, USA); P-ERK1/2 antibody (rabbit source; Abcam, Waltham, MA, USA); CD80 (B7-1) Monoclonal Antibody, PE (eBioscience, San Diego, CA, USA); CD86 (B7-2) Monoclonal Antibody, PE (eBioscience, San Diego, CA, USA); MHC Class I (H-2Kb) monoclonal antibody, PE (eBioscience, San Diego, CA, USA); MHC Class II (I-A/I-E) monoclonal antibody, PE (eBioscience); Cell Counting Kit (Beyotime Biotechnology Co., Ltd., Haimen, China); rat interleukin 4 (IL-4) ELISA (Jiangsu Jingmei Bioengineering Co., Ltd., Guangzhou City, China); rat interleukin 12 (IL-12) ELISA kit (Jiangsu Jingmei Bioengineering Co., Ltd., Guangzhou City, China).
Zhuang Z., Chen Y., Zheng J, & Chen S. (2024). The role of TRIF protein in regulating the proliferation and antigen presentation ability of myeloid dendritic cells through the ERK1/2 signaling pathway in chronic low-grade inflammation of intestinal mucosa mediated by flagellin-TLR5 complex signal. PeerJ, 12, e16716.
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4

Porcine Peripheral Blood Lymphocyte Isolation and Characterization

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Porcine peripheral blood lymphocyte separation kit and red blood cell lysing buffer were purchased from Solarbio Science & Technology (Beijing) Co., Ltd. Enzyme-Linked Immunosorbent Assay (ELISA) kits were purchased from Dongge Biological (Beijing) Co., Ltd. Mouse Anti-Porcine CD21 IgG was purchased from Southern Biotech (Alabama). Anti-FITC MicroBeads and MiniMACS Starting kits were purchased from Miltenyi Biotec (Bergisch-Gladbach). Anti-CD54/ICAM1 antibody was purchased from Arigo (Taiwan). Mouse IgG1 kappa Isotype Control was purchased from Invitrogen (Waltham). Goat anti-Pig IgM was purchased from Bio-Rad (California). CD80 (B7-1) Monoclonal Antibody was purchased from eBioscience (California). Mouse anti-Pig-SLA Class II DR and mouse anti-Mouse MHC Class I were purchased from AbD Serotec (Kidlington).
Yuan C., Zhao X., Feng Y., Chen L., Lin Y., Li T, & Song Q. (2024). Comparison of B cells' immune response induced by PEDV virulent and attenuated strains. Frontiers in Microbiology, 15, 1344344.
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