Cells were transfected with 50 nM siRNA. GLI1 siRNAs and control siRNAs were purchased from Sigma-Aldrich, ERα siRNA (sc-44204) was purchased from Santa Cruz Biotechnology. Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen) was used according to the manufacturer's protocol.
Gli1 sirnas
Manufactured by Merck Group
GLI1 siRNAs are synthetic, double-stranded RNA molecules designed to target and silence the expression of the GLI1 gene. GLI1 is a key transcription factor in the Hedgehog signaling pathway, which is involved in various cellular processes. The siRNAs are intended for use in research applications to study the role of GLI1 in cellular functions and pathways.
Automatically generated - may contain errors
2 protocols using gli1 sirnas
1
Knockdown of GLI1 and ERα in Cells
2
Modulating S6K1 and GLI1 Signaling
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siRNAs against S6K1 (RPS6KB1) (NCBI Reference Sequence: NM_003161.3) were designed and ordered from Dharmacon (SiGenome SMART pools, Thermo Scientific). GLI1 siRNAs and control siRNAs were purchased from Sigma-Aldrich.
S6K1 overexpression plasmids, wild type plasmid S6K1WT, constitutively activated plasmid S6K1T389E and function-loss plasmid S6K1T389A were kind gifts of Mien-Chie Hung (University of Texas, MD Anderson Cancer Center, Houston, TX). The GLI1 expression construct (Flag-tagged) has been described previously [15 (link)].
S6K1 overexpression plasmids, wild type plasmid S6K1WT, constitutively activated plasmid S6K1T389E and function-loss plasmid S6K1T389A were kind gifts of Mien-Chie Hung (University of Texas, MD Anderson Cancer Center, Houston, TX). The GLI1 expression construct (Flag-tagged) has been described previously [15 (link)].
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