Fetal bovine serum (FBS), trypsin, Dulbecco's modified Eagle's medium (DMEM) and Lipofectamine 2000 were purchased from Invitrogen, USA. PCR primers, Taq DNA polymerase, DNA ladder and oligo(dT)s were obtained from Sangon, China. The PmeI, PacI, and HindIII restriction enzymes were purchased from NEB. The plasmid DNA extraction (Mini) kit was obtained from QIAGEN, UK. The Escherichia coli strain DH5a and the AdEasy Vector System were purchased from GeneChem, China. HEK293T cells (ATCC#: CRL-11268) were used to generate adenoviral particles. Sprague-Dawley rats were obtained from the Experimental Animal Center of Tongji Medical College and used in the following protocols approved through the Animal Care and Use Committee of Tongji Medical College of Huazhong University of Science and Technology (Permit Number: 20051007).
Dna ladder
Manufactured by Sangon
Sourced in China
A DNA ladder is a tool used in molecular biology and genetics to determine the size of DNA fragments. It consists of a set of DNA fragments of known lengths that are used as a reference to estimate the size of unknown DNA samples when run alongside them on a gel electrophoresis.
Automatically generated - may contain errors
Lab products found in correlation
Hek293t cell, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Hindiii, by New England Biolabs (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Pcr primers, by Sangon (1 mentions)
Taq dna polymerase, by Sangon (1 mentions)
Agarose, by Beyotime (1 mentions)
Sds page gel preparation kit, by Beyotime (1 mentions)
Acrylamide mix solution, by Sangon (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Oligonucleotide, by Sangon (1 mentions)
Hela cells, by Procell (1 mentions)
Kanamycin sulfate, by Sangon (1 mentions)
Protein loading buffer, by Sangon (1 mentions)
Tryptone, by Thermo Fisher Scientific (1 mentions)
Dpni, by Thermo Fisher Scientific (1 mentions)
Easypure hipure plasmid maxiprep kit, by Transgene (1 mentions)
Yeast extract, by Thermo Fisher Scientific (1 mentions)
E coli bl21 de3 chemically competent cell, by Transgene (1 mentions)
Isopropyl β d thiogalactoside iptg, by Sangon (1 mentions)
4 protocols using dna ladder
1
Adenoviral Particle Generation in HEK293T Cells
2
DNA Synthesis and Purification Protocol
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DNAs utilized in this study were synthesized and HPLC purified by Sangon Biotechnology Co., Ltd. (Shanghai, China). The DNA sequences are listed in Table S1.† The stock solution of each DNA (100 μM) was prepared with TE buffer containing 10 mM Tris–HCl, 1 mM EDTA, and 12.5 mM MgCl2 (pH 7.4). The 40% acrylamide mix solution, ammonium persulfate (APS), 1,2-bis(dimethylamino)- ethane (TEMED), and DNA ladder were acquired from Sangon Biotechnology Co., Ltd. (Shanghai, China). All the chemicals were of analytical grade and utilized as received without further purification. All oligonucleotides were purchased from Sangon Biotechnology Co., Ltd. (Shanghai, China). All sequencing experiments were performed at Sangon Biotechnology Co., Ltd. (Shanghai, China). DFHBI-1 T (MedChemExpress) and BI were synthesized by our laboratory, SDS-PAGE gel preparation kit, DAPI, and agarose were obtained from Beyotime (Shanghai, China). The HeLa cells (human cervical carcinoma cell line), and CHO cells (Chinese Hamster Ovary) were purchased from Procell, Inc.
3
Aspergillus terreus At-ATA cDNA Cloning
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The At-ATA cDNA from Aspergillus terreus sequence, including the NcoI and XhoI restriction sites, was synthesized by General Biosystems (Chuzhou, China), and the plasmid pET-28a(+) was used for gene cloning and DNA sequencing. All PCR primers were synthesized by Qingke Biology Co., Ltd. (Hangzhou, China). PrimeSTAR® Max DNA polymerase was obtained from Takara Biotechnology (Dalian, China) for the polymerase chain reaction (PCR). Dpn I, Yeast extract and tryptone were obtained from Thermo Fisher Scientific (Shanghai, China). Dimethyl sulfoxide (DMSO), 1-(R)-PEA and pyruvate were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). NaCl, NaH2PO4, Na2HPO4, NaOH, DNA ladder, protein marker, protein loading buffer, kanamycin sulfate, isopropyl-β-d-thiogalactoside (IPTG), Ni-NTA Sefinose (TM) Resin (Settled Resin) kit, SDS-PAGE gel kit, and Modified Bradford Protein Assay Kit were obtained from Sangon (Shanghai, China). E. coli BL21(DE3) Chemically Competent Cell, EasyPure® HiPure Plasmid MaxiPrep Kit, EasyPure® Quick Gel Extraction Kit and EasyPure® PCR Purification Kit were purchased from TransGen Biotech (Beijing, China).
4
Molecular Cloning and Verification
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Restrictions enzymes, T4 DNA ligase, and DNA Ladder were purchased from Sangon (Shanghai, China). PrimeS-TAR™ HS DNA polymerase was purchased from TaKaRa (Dalian, China). Plasmid Minipreps Purification System B used for isolating the plasmid DNA from E. coli was from PCR experiments were performed using Mastercycler from Eppendorf (Hamburg, Germany). The sequences of all primers used in this study are listed in Table 2.
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