Malondialdehyde bis dimethyl acetal

N^α-(tert-butoxycarbonyl)-L-Lys (Boc-Lys), ε-aminocaproic acid (6-ACA), AA, FA, BSA, trifluoroacetic acid, 3,3′,5,5′-tetramethylbenzidine (TMB), human MPO, and H₂O₂ were purchased from Sigma-Aldrich (St. Louis, MO). Malondialdehyde bis(dimethyl) acetal, Dynabeads M-270 Amine, CarboxyLink Kit, the Imject EDC mcKLH Spin Kit, goat anti-rabbit IgG (H + L) antibody with HRP, goat anti-human IgG/IgM secondary antibody, and goat anti-mouse IgM secondary antibody were obtained from Thermo Scientific (Rockford, IL). Protein A column and autoLDL™ Cholesterol kit were purchased from GE Healthcare Life Sciences (Pittsburgh, PA) and Pointe Scientific (Lincoln Park, MI), respectively. EnVision + Single Reagents anti-mouse/rabbit-HRP were from Dako North America, Inc. (Carpentaria, CA). The anti-M2AA mouse monoclonal antibody (1F83)²⁴ and rabbit polyclonal antibody^{48 (link)} were provided by Drs. Koji Uchida (Nagoya University) and Todd A. Wyatt (University of Nebraska), respectively.

Boc-lysine, 6-ACA, acetaldehyde, BSA, KLH, TFA, TMB, and H₂O₂ were purchased from Sigma-Aldrich (St. Louis, MO). Malondialdehyde bis(dimethyl acetal), the Imject EDC mcKLH Spin Kit, goat anti-rabbit IgG (H+L) antibody with HRP, and goat anti-mouse IgM secondary antibody were obtained from Thermo Scientific (Rockford, IL). EnVision+Single Reagents anti-mouse-HRP and rabbit anti-human IgG F(ab’)2 fragment antibody with HRP were purchased from Dako North America, Inc. (Carpentaria, CA) and Jackson Immuno Research Laboratories (West Grove, PA), respectively.