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Nbp2 42379

Manufactured by Novus Biologicals
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NBP2–42379 is a laboratory instrument that is used for the detection and quantification of target molecules in biological samples. The core function of this product is to perform analytical procedures, such as immunoassays or spectrophotometric analysis, to measure the presence and concentration of specific analytes.

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Lab products found in correlation

Er enrichment kit, by Novus Biologicals (1 mentions) Af5012, by Beyotime (1 mentions)

2 protocols using nbp2 42379

1

ER Microsome Trypsinization Assay

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ER microsomal fraction (300 μl) was collected from 0.4 g cell pellet by ER enrichment kit (NBP2–29482, Novus Biologicals, Littleton, CO). After pretreatment with or without 1% triton X100 for 3 min, trypsin/EDTA (0.625 g/L trypsin and 0.05 g/L EDTA in PBS) solution was added to each ER microsomal fraction (20 μl). Samples were mixed and incubated for the indicated time. After trypsinization, mixed samples were subjected to Western blot analysis using primary antibodies against IRE1α (3294, 1:2000; Cell Signaling Technology), HSP90B1 (NBP2–42379, 1:3000; Novus), and AMPKα (5831S, 1:2000; Cell Signaling Technology).
Cha J.H., Yang W.H., Xia W., Wei Y., Chan L.C., Lim S.O., Li C.W., Kim T., Chang S.S., Lee H.H., Hsu J.L., Wang H.L., Kuo C.W., Chang W.C., Hadad S., Purdie C.A., McCoy A.M., Cai S., Tu Y., Litton J.K., Mittendorf E.A., Moulder S.L., Symmans W.F., Thompson A.M., Piwnica-Worms H., Chen C.H., Khoo K.H, & Hung M.C. (2018). Metformin promotes antitumor immunity via endoplasmic reticulum-associated degradation of PD-L1. Molecular cell, 71(4), 606-620.e7.
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2

Isolation and Analysis of ER Proteins

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ER fraction was collected from pancreatic cancer cells by ER enrichment kit (NBP2-29482, Novus Biologicals). After pretreatment with or without 1%, Triton X-100 for 3 min, the solution (0.625 g/L trypsin + 0.05 g/L EDTA in PBS) was added to ER fraction. Samples were incubated for the indicated time. After trypsinization, samples were analyzed by Western blotting with primary antibodies against IRE1α (3294, Cell Signaling Technology), HSP90B1 (NBP2-42379, Novus Biologicals), EGFR (4267T, Cell Signaling Technology), NEK2 (24171-1-AP, Proteintech Group), and a-Tubulin (AF5012, Beyotime).
Zhang X., Huang X., Xu J., Li E., Lao M., Tang T., Zhang G., Guo C., Zhang X., Chen W., Yadav D.K., Bai X, & Liang T. (2021). NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1. Nature Communications, 12, 4536.
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