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Bac tracker supercoiled dna ladder

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The Bac-Tracker Supercoiled DNA ladder is a laboratory equipment product used for the identification and size determination of supercoiled DNA molecules in agarose gel electrophoresis. It provides a reference scale to assess the molecular weight of supercoiled DNA samples.

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Lab products found in correlation

Plasmid purification mini kit, by Qiagen (1 mentions)

2 protocols using bac tracker supercoiled dna ladder

1

Plasmid DNA Isolation and Characterization

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Plasmid DNA was isolated by the method described by Kado and Liu [24] (link) followed by phenol–chloroform extraction. The Bac-Tracker Supercoiled DNA ladder (Epicentre Biotechnologies, Madison, WI, USA) and a 94-kb plasmid from S. Typhimurium LT2 were used as size markers.
Ido N., Lee K.I., Iwabuchi K., Izumiya H., Uchida I., Kusumoto M., Iwata T., Ohnishi M, & Akiba M. (2014). Characteristics of Salmonella enterica Serovar 4,[5],12:i:- as a Monophasic Variant of Serovar Typhimurium. PLoS ONE, 9(8), e104380.
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2

Plasmid Purification and Replicon Typing

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Plasmid DNA was purified using a Plasmid mini purification kit (Qiagen Inc., CA, USA). The BAC-Tracker supercoiled DNA ladder (Epicentre Biotechnologies Inc., WI, USA) was used as a size marker for plasmid analysis. Plasmids were analyzed using the PCR-based replicon typing method to identify the plasmid type [15 (link)]. All detected replicon types were confirmed by sequencing.
Lee K.E., Lim S.I., Choi H.W., Lim S.K., Song J.Y, & An D.J. (2014). Plasmid-mediated AmpC β-lactamase (CMY-2) gene in Salmonella typhimurium isolated from diarrheic pigs in South Korea. BMC Research Notes, 7, 329.
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