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7500 and 7500 fast real time pcr systems

Manufactured by Thermo Fisher Scientific
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The 7500 and 7500 Fast Real-Time PCR Systems are high-performance real-time PCR instruments designed for accurate and sensitive nucleic acid detection and quantification. The systems use 96-well or 384-well plates and provide real-time monitoring of amplification. The 7500 and 7500 Fast systems are capable of running a wide range of real-time PCR applications.

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Lab products found in correlation

Chamq sybr qpcr master mix, by Thermo Fisher Scientific (3 mentions) Rneasy mini kit, by Qiagen (1 mentions) Reverse transcription system, by Promega (1 mentions) 7500 real time pcr system, by Thermo Fisher Scientific (1 mentions) Taqman universal pcr master mix, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

7500 Real-Time PCR System (4866 citations) 7500 Fast Real-Time PCR System (4300 citations) Fast Real-Time PCR System (114 citations) 7500 Real-Time System (97 citations) 7500 Fast Real-Time PCR (92 citations) 7500 Fast Real-Time System (60 citations) 7500 Fast & 7500 Real-Time PCR System (6 citations) 7500 Fast Real PCR System (4 citations) 7500 Fast and 7500 Real-Time PCR System (2 citations) 7500 Fast System real-time PCR system (2 citations)

4 protocols using 7500 and 7500 fast real time pcr systems

1

RNA Extraction and RT-qPCR Analysis

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Total RNA was extracted and purified with FastPure Plant Total RNA Isolation Kit and reverse transcription was employed using HiScript II Q Select RT SuperMix for qPCR. RT-qPCR was performed on Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems using ChamQ SYBR qPCR Master Mix. PCR cycling conditions were 95 °C for 30 s, followed by 40 cycles of 95 °C for 10 s, 63 °C for 10 s and 72 °C for 30 s. The cDNA melting curve was set as usual. Relative gene expression was calculated using 2−ΔΔCT method, calibrated against GAPDH or beta Actin.
Pan B., Wang Z., Zhang X., Shen S., Ke X., Qiu J., Yao Y., Wu X., Wang X, & Tang N. (2023). Targeted inhibition of RBPJ transcription complex alleviates the exhaustion of CD8+ T cells in hepatocellular carcinoma. Communications Biology, 6, 123.
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2

RNA Extraction and RT-qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted and purified with FastPure Plant Total RNA Isolation Kit and reverse transcription was employed using HiScript II Q Select RT SuperMix for qPCR. RT-qPCR was performed on Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems using ChamQ SYBR qPCR Master Mix. PCR cycling conditions were 95 °C for 30 s, followed by 40 cycles of 95 °C for 10 s, 63 °C for 10 s and 72 °C for 30 s. The cDNA melting curve was set as usual. Relative gene expression was calculated using 2−ΔΔCT method, calibrated against GAPDH or beta Actin.
Pan B., Wang Z., Zhang X., Shen S., Ke X., Qiu J., Yao Y., Wu X., Wang X, & Tang N. (2023). Targeted inhibition of RBPJ transcription complex alleviates the exhaustion of CD8+ T cells in hepatocellular carcinoma. Communications Biology, 6, 123.
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3

Quantification of Cytokine mRNA Expression

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Cells were prepared in the same manner as described above. Total RNA was isolated and purified using RNeasy Mini Kit (Qiagen, Valencia, CA) and reverse transcribed into cDNA using the Reverse Transcription System (Promega, Madison, WI) according to the protocols provided by the manufacturers. IL-1β, IL-6, IL-8 and CCL5 mRNA levels were assessed using a 7500 Real Time PCR System (Applied Biosystems, Foster City, CA) and TaqMan Universal PCR Master Mix. The primers and probes were obtained from Applied Biosystems. The following thermocycling conditions were used: 50°C for 2 min, followed by 95°C for 10 min, 95°C for 15 s, and 60°C for 1 min for 40 cycles. The threshold cycle (CT) from each well was determined using 7500 Software v2.0.5 for Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems. Expression of mRNA was calculated by the comparative CT method. PCR amplification of GAPDH (housekeeping gene) was performed for each sample to normalize mRNA levels of the target genes.
Wrobel J.K., Choi J.J., Xiao R., Eum S.Y., Kwiatkowski S., Wolff G., Spangler L., Power R.F, & Toborek M. (2014). SELENOGLYCOPROTEINS ATTENUATE ADHESION OF TUMOR CELLS TO THE BRAIN MICROVASCULAR ENDOTHELIUM VIA A PROCESS INVOLVING NF-κB ACTIVATION. The Journal of nutritional biochemistry, 26(2), 120-129.
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4

RNA Extraction and RT-qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted and purified with FastPure Plant Total RNA Isolation Kit and reverse transcription was employed using HiScript II Q Select RT SuperMix for qPCR. RT-qPCR was performed on Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems using ChamQ SYBR qPCR Master Mix. PCR cycling conditions were 95 °C for 30 s, followed by 40 cycles of 95 °C for 10 s, 63 °C for 10 s and 72 °C for 30 s. The cDNA melting curve was set as usual. Relative gene expression was calculated using 2−ΔΔCT method, calibrated against GAPDH or beta Actin.
Pan B., Wang Z., Zhang X., Shen S., Ke X., Qiu J., Yao Y., Wu X., Wang X, & Tang N. (2023). Targeted inhibition of RBPJ transcription complex alleviates the exhaustion of CD8+ T cells in hepatocellular carcinoma. Communications Biology, 6, 123.
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