Annexin 5 binding

Manufactured by Thermo Fisher Scientific

Annexin-V is a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine. It is commonly used as a marker for detecting apoptosis (programmed cell death) in various cell types. The Annexin-V binding assay is a tool for the detection and quantification of apoptotic cells.

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Lab products found in correlation

Annexin 5 binding buffer, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Annexin V (493 citations) Annexin V binding buffer (107 citations) Annexin-V binding buffer (1X) (3 citations) Annexin-V-FITC binding buffer (2 citations) Annexin V-FITC binding solution (2 citations) 1X Annexin-V Binding Buffer (ABB) (2 citations)

2 protocols using annexin 5 binding

Annexin-V Binding Assay for Apoptosis

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Apoptotic and dead cells in culture were detected by Annexin-V binding assay using Alexa Fluor 594 conjugated Annexin-V (Invitrogen) and Annexin-V binding buffer (Invitrogen) according to manufacturer’s instructions. Fluorescent images were recorded as describe above.

Kido T., Li Y., Tanaka Y., Dahiya R, & Chris Lau Y.F. (2019). The X-linked tumor suppressor TSPX downregulates cancer-drivers/oncogenes in prostate cancer in a C-terminal acidic domain dependent manner. Oncotarget, 10(15), 1491-1506.

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Apoptosis Assay of VEGFD-SK Cells

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VEGFD-SK cells (2×10⁵/well) were seeded in 6-well dishes and incubated to 80% confluence. pVAX:lip (containing 2 μg/ml pVA plasmid) or pVAX-MP:lip (containing 2 μg/ml pVAX-MP plasmid) was prepared in 2 ml RPMI-1640 medium without serum. VEGFD-SK cells were incubated with the complexes for 6 h. Then, VEGFD-SK cells were supplemented with medium containing serum for further 48 h. Untreated VEGFD-SK cells were needed as control. Quantitative evaluation of apoptotic VEGFD-SK cells was performed by flow cytometric analysis after Annexin V binding and propidium iodide staining (Invitrogen).

QI X., DU L., CHEN X., CHEN L., YI T., CHEN X., WEN Y., WEI Y, & ZHAO X. (2016). VEGF-D-enhanced lymph node metastasis of ovarian cancer is reversed by vesicular stomatitis virus matrix protein. International Journal of Oncology, 49(1), 123-132.

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