Rats were anesthetized with an intraperitoneal injection of ketamine HCl (95.0 mg/kg; Vedco), xylazine (10.0 mg/kg; Lloyd Laboratories), and acepromazine (0.075 mg/kg; Phoenix Pharm Inc). Using a #11 surgical blade (Electron Microscopy Sciences), a one-inch midline incision was made on the dorsal surface of skin and muscle to expose the dorsal surface of the C2 and C3 vertebrae. A laminectomy was performed above C2 to expose the spinal cord using rongeurs (Fine Science Tools). A hemisection was performed just caudal to the C2 root with a dissecting knife (Fine Science Tools; Urban et al., 2018 (link)). To ensure a complete hemisection, a 30-gauge needle (BD Biosciences) was passed through the injury several times.
Dissecting knife
Manufactured by Fine Science Tools
The Dissecting Knife is a sharp, fine-tipped blade designed for precision cutting and dissection tasks in laboratory and scientific settings. It features a sturdy, ergonomic handle and a high-quality stainless steel blade for accurate and controlled incisions.
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Lab products found in correlation
2 protocols using dissecting knife
1
Cervical Spinal Cord Hemisection in Rats
2
Microvelia Leg Ablation Protocol
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Before a particular Microvelia was ablated, it was anesthetized by placing it into a freezer for approximately 2 minutes. This led to the insect’s temporary incapacitation, which allowed for easier and more accurate ablation to be done. After being taken out of the freezer, we placed the Microvelia under a magnifying glass, and the according segment(s) (Fig1.c ) of the leg(s) were removed with a Fine Science Tools dissecting knife. An example of a Microvelia with its middle tarsi ablated is shown in Fig1.a . After being cut, the specimen would reawaken and be placed into a small container of water from its natural habitat for recovery. After one hour of being in the container, the Microvelia was removed, its locomotion was recorded, and it was then placed back into containment. Additionally, after having 24 hours to recuperate from the initial ablation, the insect’s locomotion was once again recorded.
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