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Horseradish peroxidase conjugated anti mouse and anti rabbit antibodies

Manufactured by GE Healthcare
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Sourced in Germany

Horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies are laboratory reagents used in various immunoassay techniques, such as Western blotting and ELISA. These antibodies are conjugated with the enzyme horseradish peroxidase, which is capable of catalyzing a colorimetric or chemiluminescent reaction, allowing for the detection and visualization of target proteins or molecules.

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Lab products found in correlation

Protein assay kit, by Bio-Rad (1 mentions)

Close spelling variants of this product

Anti-rabbit and anti-mouse horseradish-peroxidase-conjugated secondary antibodies Anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies Horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit IgG antibodies Horseradish peroxidase-conjugated anti-rabbit and anti-mouse IgG antibodies Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies Donkey anti-rabbit and sheep anti-mouse horseradish peroxidase–conjugated secondary antibodies Horseradish peroxidase-conjugated donkey anti-rabbit and sheep anti-mouse antibodies Horseradish peroxidase-conjugated anti-rabbit and anti-mouse Horseradish peroxidase

2 protocols using horseradish peroxidase conjugated anti mouse and anti rabbit antibodies

1

CASK Protein Detection Assay

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The following primary antibodies and dilutions were used: rabbit anti-CASK antibody (named anti-CASK2 antibody; WB 1:500; Cell Signaling Technology, Danvers, MA, USA), mouse anti-CASK antibody (named anti-CASK1 antibody; WB 1:1000; Millipore, Schwalbach, Germany), mouse anti-α-tubulin antibody (clone DM 1A; WB 1:7500; Sigma-Aldrich, Taufkirchen, Germany). As secondary antibodies, horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:1000–1:10000 dilution; GE Healthcare, Munich, Germany) were used.
Moog U., Bierhals T., Brand K., Bautsch J., Biskup S., Brune T., Denecke J., de Die-Smulders C.E., Evers C., Hempel M., Henneke M., Yntema H., Menten B., Pietz J., Pfundt R., Schmidtke J., Steinemann D., Stumpel C.T., Van Maldergem L, & Kutsche K. (2015). Phenotypic and molecular insights into CASK-related disorders in males. Orphanet Journal of Rare Diseases, 10, 44.
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2

Western Blotting Procedure for Protein Analysis

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Western blotting was performed, as previously described (18 (link), 19 (link)). Briefly, cells were lysed with RIPA lysis buffer and centrifuged for 15 minutes at 4°C. Protein concentration was assessed using a Bio-Rad protein assay kit. The protein was loaded into gels (20 μg/well), and the bands were separated using electrophoresis. Bands were transferred to nitrocellulose membranes, blocked with 5% milk for 1 hour at ambient temperature, and incubated with primary antibodies against PACT or Dicer (1:1000 dilution) overnight at 4°C. Samples of proteins were incubated with horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies (GE Healthcare) or an anti-goat antibody (Santa Cruz Biotechnology) for 1 hour at ambient temperature. Blots were developed and analyzed, as described previously (18 (link), 19 (link)). Loading control (actin) was used; all experiments were performed in duplicate.
Hisamatsu T., McGuire M., Wu S.Y., Rupaimoole R., Pradeep S., Bayraktar E., Noh K., Hu W., Hansen J.M., Lyons Y., Gharpure K.M., Nagaraja A.S., Mangala L.S., Mitamura T., Rodriguez-Aguayo C., Eun Y.G., Rose J., Bartholomeusz G.A., Ivan C., Lee J.S., Matsuo K., Frumovitz M., Wong K.K., Lopez-Berestein G, & Sood A.K. (2018). PRKRA/PACT Expression Promotes Chemoresistance of Mucinous Ovarian Cancer. Molecular cancer therapeutics, 18(1), 162-172.
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