PBMCs were isolated using Ficoll-Paque density gradient centrifugation, frozen in freezing medium [10% DMSO, 90% fetal bovine serum (FBS)] and stored at -80°C until use. For staining, PBMCs were thawed using complete RPMI (RPMI+10%FBS) and washed with PBS. The cells were first stained using Fixable Aqua Dead Cell Kit (Thermofisher), followed by staining with anti-CD3 (UCHT1) (Biolegend), anti-CD4 (RPA-T4) (BD Biosciences), anti-CD8 (SK1) (Biolegend), anti-CD19 (HIB19) (BD Biosciences), anti-CD27 (O323) (Biolegend), anti-CD56 (B159) (BD Biosciences), anti-CD45RA (2H4) (Beckman Coulter), anti-CD38 (HIT2) (BD Biosciences) and anti-CD38 (JK36) (Beckman Coulter). The cells were washed and analyzed using BD LSRFortessa™ cell analyzer. Data analyses were performed using Flowjo V10.5.3 (BD)
Anti cd45ra 2h4
Manufactured by Beckman Coulter
The Anti-CD45RA (2H4) is a monoclonal antibody that binds to the CD45RA isoform of the CD45 protein expressed on the surface of human lymphocytes. It is used for the identification and enumeration of CD45RA+ cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 ucht1, by BioLegend (1 mentions)
Anti cd8 sk1, by BioLegend (1 mentions)
Anti cd27 o323, by BioLegend (1 mentions)
Lsrfortessa cell analyzer, by BD (1 mentions)
Anti cd4 rpa t4, by BD (1 mentions)
Anti cd56 b159, by BD (1 mentions)
Anti cd19 hib19, by BD (1 mentions)
Fixable aqua dead cell kit, by Thermo Fisher Scientific (1 mentions)
Anti cd38 hit2, by BD (1 mentions)
Anti cd45ro, by Beckman Coulter (1 mentions)
Anti γδ tcr, by Beckman Coulter (1 mentions)
Navios, by Beckman Coulter (1 mentions)
7 aminoactinomycin d, by Beckman Coulter (1 mentions)
2 protocols using anti cd45ra 2h4
1
Multiparametric Flow Cytometry Immune Profiling
2
Multiparametric Flow Cytometry Phenotyping
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Cells were harvested and phenotypically characterized by 10-color flow cytometry (Navios, Beckman Coulter) using the following antibodies: anti-CD45 (J.33), anti-CD14 (RMO52), anti-CD3 (UCHT-1), anti-CD56 (N901), anti-CD19 (J3-119), anti-CD16 (3G8), anti-CD4 (13B8.2), anti-CD8 (B9.11), anti-CD25 (B1.49.9), anti-CD314 (ON72), anti-αβ-TCR (BW242/412), anti-γδ-TCR (IMMU510), anti-CD45RO (UCHL1), anti-CD45RA (2H4) and anti-CD62L (DREG56) (Beckman Coulter; except antiαβ-TCR, Miltenyi Biotec). For the assessment of viability, 7-aminoactinomycin D (Beckman Coulter) was used, and measurements were performed using a single-platform approach. EBV-specific lymphocytes were identified by staining with various MHC-I and MHC-II antibodies using multimer technology: LMP2A (A*02:01), EBNA1 (B*35:01), EBNA3A (B*07:02 and B*08:01), BMLF1 (A*02:01; Immudex) and EBNA1 (DRB1*04:01; ProImmune).
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