Fifth-instar larvae female nymphs of the above two strains (PS and PR strain) were used for RNA extraction. Total RNA was extracted following the manufacturer’s instructions (Invitrogen Trizol Reagent of RNA extraction kit 15596018). This was carried out to ensure that the samples for transcriptome sequencing were of a sufficient quality. The purity, concentration, and integrity of RNA samples were tested according to the manufacturer’s methods for the gel and Nanodrop 2000 (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100 (Agilent).
Trizol reagent of rna extraction kit
Manufactured by Thermo Fisher Scientific
Trizol Reagent is a phenol-based RNA extraction kit used for the isolation of total RNA from a variety of sample types, including cells, tissues, and body fluids. The kit utilizes a monophasic solution of phenol and guanidine isothiocyanate to facilitate the lysis of cells and the subsequent separation of RNA from DNA and proteins.
Automatically generated - may contain errors
3 protocols using trizol reagent of rna extraction kit
1
Transcriptome Analysis of Insect Strains
2
Transcriptome Analysis of Leaf Samples
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Mixed samples of five leaves in the treatment of CK, CKL, and U3 were used for RNA extraction (Invitrogen Trizol Reagent of RNA extraction kit 15596018). A cDNA library was constructed using a NEB kit for library preparation and sequenced using an Illumina HiSeq2500 (Beijing Biomarker Technologies Co.)
3
Transcriptomic Analysis of Lvfeng 2 Treated Samples
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Lvfeng 2 was used as the experimental material. There were 6 treatments (CK1, D1 and D1 + S and CK4, D4 and D4 + S), which are described in the plant materials and UNZ treatments section. Each treatment consisted of 3 replicates. Sample collection was carried out between 8:00 and 9:00 a.m. Mixed samples of three leaves each in the CK, D and D + S treatments were subjected to RNA extraction (Invitrogen TRIzol Reagent of RNA extraction kit 15,596,018). A total of eighteen libraries were constructed using a NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) and sequenced using an Illumina HiSeqTM2000 (Beijing Biomarker Technologies Co.).
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