To determine the EPC-EX uptake mechanisms by ASCs, we focused on the endocytic uptake pathways, including macropinocytosis, clathrin and caveolin-dependent pathways. For this, the cells were pre-treated for 30 min with various inhibitors: 80 μM dynasore (dynamin inhibitor, Sigma-Aldrich), 5 μM LY290042 (macropinocytosis inhibitor, Enzo), 10 μM pitstop 2 (clathrin inhibitor, Abcam) or 200 μM genistein (caveolin inhibitor, EMD Millipore). The concentration of the drugs was determined by following our previous studies [24 (link)]. After treatment, the cells were washed twice with PBS and then the PKH-labelled EPC-EXs were co-incubated with the ASCs for 24 h. Fluorescent images were obtained by a fluorescence microscope (EVOS, NY) and fluorescent intensity was quantified by flow cytometry analysis (Accuri C6 flow cytometer).
Ly290042
Manufactured by Enzo Life Sciences
LY290042 is a laboratory reagent manufactured by Enzo Life Sciences. It is a selective inhibitor of the enzyme phosphoinositide 3-kinase (PI3K). The core function of LY290042 is to inhibit PI3K activity, which is commonly used in research applications to study signal transduction pathways involving PI3K.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ly290042
1
Investigating EPC-EX Uptake Mechanisms in ASCs
2
Uptake Mechanisms of EPC-Derived Exosomes by ASCs
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To determine the EPC-EX uptake mechanisms by ASCs, we focused on the endocytic uptake pathways, including macropinocytosis, clathrin and caveolin-dependent pathways. For this, the cells were pre-treated for 30 minutes with various inhibitors: 80 μM dynasore (dynamin inhibitor, Sigma-Aldrich), 5 μM LY290042 (macropinocytosis inhibitor, Enzo), 10 μM pitstop 2 (clathrin inhibitor, Abcam) or 200 μM genistein (caveolin inhibitor, EMD Millipore). After treatment, the cells were washed twice with PBS and then the PKH-labelled EPC-EXs were co-incubated with the ASCs for 24 hours. Fluorescent images were obtained by a uorescence microscope (EVOS, NY) and uorescent intensity was quanti ed by ow cytometry analysis (Accuri C6 ow cytometer).
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