Molar mass was determined using size-exclusion chromatography coupled with multi-angle laser light scattering (SEC-MALLS). Samples (2 mg/mL in 0.1 mol L -1 NaNO3) were allowed to hydrate by standing at room temperature overnight and centrifuged (14,000 x g, 10 min) to clarify. The soluble material (100 µL) was injected onto two columns (TSK-Gel G5000PWXL and G4000PWXL, 300 x 7.8mm, Tosoh Corp., Tokyo, Japan) connected in series and eluted with 0.1 M NaNO3 (0.7 mL min -1 , 60 C). The eluted material was detected using a UV spectrophotometer (280 nm), a DAWN-EOS MALLS detector (Wyatt Technology Corp., Santa Barbara, USA) and a refractive index (RI) monitor (Waters Corp., Milford, USA). The data for molar mass determination was analysed using ASTRA software (v6.1.84, Wyatt Technology Corp.) using a refractive index increment, dn/dc, of 0.146 mL g -1 [24] .
Refractive index monitor
Manufactured by Waters Corporation
Sourced in United States
The Refractive Index (RI) monitor is a laboratory instrument designed to measure the refractive index of a fluid or liquid sample. It operates by directing a beam of light through the sample and detecting the angle at which the light is refracted, which is directly related to the refractive index of the material. The RI monitor provides precise and reliable measurements of the refractive index, which is a fundamental property of a substance and is often used in various scientific and industrial applications.
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2 protocols using refractive index monitor
1
Molecular Weight Analysis of Polymers
2
Molecular Weight Analysis of Polymers
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Molar mass was determined using size-exclusion chromatography coupled with multi-angle laser light scattering (SEC-MALLS). Samples (2 mg/mL in 0.1 mol L -1 NaNO3) were allowed to hydrate by standing at room temperature overnight and centrifuged (14,000 x g, 10 min) to clarify. The soluble material (100 µL) was injected onto two columns (TSK-Gel G5000PWXL and G4000PWXL, 300 x 7.8mm, Tosoh Corp., Tokyo, Japan) connected in series and eluted with 0.1 M NaNO3 (0.7 mL min -1 , 60 C). The eluted material was detected using a UV spectrophotometer (280 nm), a DAWN-EOS MALLS detector (Wyatt Technology Corp., Santa Barbara, USA) and a refractive index (RI) monitor (Waters Corp., Milford, USA). The data for molar mass determination was analysed using ASTRA software (v6.1.84, Wyatt Technology Corp.) using a refractive index increment, dn/dc, of 0.146 mL g -1 [24] .
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