POPC and DGS-NTA-Ni were purchased from Avanti Polar Lipids Inc. Texas Red–DHPE, NeutrAvidin, tris-HCl (tris hydrochloride), Hepes, IPTG (isopropyl-β-d -thiogalactopyranoside), b-mercaptoethanol, TCEP [tris(2-carboxyethyl)phosphine], and Triton X-100 were purchased from Thermo Fisher Scientific. Tryptone, yeast extract, NaCl, NaH2PO4, Na2HPO4, urea, sodium tetraborate, EDTA, CaCl2, glycerol, EDTA-free protease inhibitor tablets, imidazole, PMSF (phenylmethanesulfonylfluoride), PLL (poly-l -lysine), Atto 594 NHS-ester, and Atto 488 NHS-ester were purchased from Sigma-Aldrich. DP-EG10-biotin was provided by D. Sasaki from Sandia National Laboratories, Livermore, CA (40 (link)). Amine-reactive polyethylene glycol [mPEG-succinimidyl valerate (SVA); molecular weight (MW), 5000] and PEG-biotin (Biotin-PEG SVA; MW, 5000) were purchased from Laysan Bio Inc. All reagents were used without further purification.
Texas red dhpe
Manufactured by Avanti Polar Lipids
Sourced in United States
Texas Red–DHPE is a fluorescent lipid probe designed for use in biological membranes. It consists of the Texas Red fluorophore covalently attached to the phospholipid 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE). This probe can be used to label and visualize lipid bilayers and cellular membranes.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Thermo Fisher Scientific (1 mentions)
Yeast extract, by Merck Group (1 mentions)
Edta free protease inhibitor tablet, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Neutravidin, by Thermo Fisher Scientific (1 mentions)
Tris hcl, by Thermo Fisher Scientific (1 mentions)
Imidazole, by Merck Group (1 mentions)
Atto 488 nhs ester, by Merck Group (1 mentions)
Tryptone, by Merck Group (1 mentions)
Atto 594 nhs ester, by Merck Group (1 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
Dgs nta ni, by Avanti Polar Lipids (1 mentions)
Polybead carboxylate microspheres, by Polysciences (1 mentions)
Fluoresbrite yellow green carboxylate microspheres, by Polysciences (1 mentions)
D glucose, by Fujifilm (1 mentions)
Sucrose, by Fujifilm (1 mentions)
Liquid paraffin, by Fujifilm (1 mentions)
2 protocols using texas red dhpe
1
Reconstitution of Membrane-Bound Proteins
2
Preparation of Giant Vesicle Membrane
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1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and TexasRed® tagged 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (TexasRed® DHPE), were purchased from Avanti Polar Lipids (AL, USA) and Invitrogen (CA, USA), respectively. The GV membrane composition was DOPC: TexasRed® DHPE = 99.7:0.3 (mol%). Polybead® carboxylate microspheres (R = 1.00 μm; 2.5 vol%; 1.05 g/cm3) and Fluoresbrite® yellow green carboxylate microspheres (R = 1.00 μm; 2.5 vol%; 1.05 g/cm3; λex, 441 nm; λem, 486 nm) were purchased from Polysciences, Inc. (PA, USA). D-Glucose, sucrose, and liquid paraffin were purchased from Wako Pure Chemical Industries (Osaka, Japan). The Tris-HCl buffer stock solution (100 mM, pH 7.5, 1.34 g/cm3) was prepared by adjusting pH of Tris (hydroxymethyl)-aminomethane (6.057 g, 50 mmol) aqueous solution to 7.5 with 1 M HCl.
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