All biotinylated and fluorescent antibodies, human CD138 microbeads, and Pan T Cell Isolation Kits were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). DMEM, RPMI-1640, L-glutamine, penicillin–streptomycin, and phosphate buffered saline (PBS) were purchased from Corning (Corning, NY). Fetal bovine serum, live-cell dyes, lipophilic tracers, collagenase, and counting beads were purchased from Life Technologies (Carlsbad, CA). 1,2-dipalmitoyl-sn- glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N- [amino(polyethylene glycol)-2000] (DSPE-PEG2000), and polycarbonate membranes were purchased from Avanti Polar Lipids (Alabaster, AL). Cholesterol and chloroform were purchased from Millipore Sigma (Burlington, MA). Streptavidin conjugation kit was purchased from Abcam (Cambridge, UK). Human Cytokine Array Q1 was purchased from RayBiotech (Peachtree Corners, GA). All mice used in this study were NCG (strain: 572), female, 50–56 days old, and purchased from Charles River (Wilmington, MA). All mice experiments in this study were in compliance with the Institutional Animal Care and Use Committee at Washington University.
Cholesterol and chloroform
Manufactured by Merck Group
Cholesterol and chloroform are laboratory reagents commonly used in various biochemical and analytical procedures. Cholesterol is a lipid molecule that plays a crucial role in cellular function, while chloroform is a volatile organic solvent. These substances are often employed in applications such as lipid extraction, purification, and analysis. However, a detailed description of their core function without extrapolation on intended use cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Counting beads, by Thermo Fisher Scientific (2 mentions)
Collagenase, by Thermo Fisher Scientific (2 mentions)
Lipophilic tracers, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Human cd138 microbead, by Miltenyi Biotec (2 mentions)
Streptavidin conjugation kit, by Abcam (2 mentions)
Human cytokine array q1, by RayBiotech (2 mentions)
Pan t cell isolation kit, by Miltenyi Biotec (2 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions)
Polycarbonate membrane, by Avanti Polar Lipids (2 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n amino polyethylene glycol 2000 dspe peg2000, by Avanti Polar Lipids (2 mentions)
Penicillin streptomycin, by Corning (2 mentions)
Dulbecco modified eagle medium (dmem), by Corning (2 mentions)
L glutamine, by Corning (2 mentions)
Rpmi 1640, by Corning (2 mentions)
2 protocols using cholesterol and chloroform
1
Isolation and Characterization of Immune Cells
2
Isolation and Analysis of Tumor-Infiltrating Cells
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