Methicillin-resistant Staphylococcus aureus (MRSA) strain ATCC 43300 and Pseudomonas aeruginosa strain ATCC 27853 (Microbiologics, USA) were used in this study.
Atcc43300
Manufactured by Microbiologics
Sourced in United States
ATCC43300 is a laboratory strain of Escherichia coli, a common bacterium. It is a well-characterized strain that is commonly used in research and testing applications.
Automatically generated - may contain errors
5 protocols using atcc43300
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MRSA and Pseudomonas aeruginosa Strains
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Chitosan-Based Antimicrobial Formulations
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Commercial chitosan with a deacetylation degree around 75% (lot STBF8219V) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The anionic polyelectrolytes corresponding to the sodium and potassium salts of poly(maleic acid-alt-ethylene) (PAM-2Na or PAM-2K) and poly(maleic acid-alt-octadecene) (PAM-18Na or PAM-18K) previously synthesized and characterized [31 (link),32 (link)], were provided by the Laboratory of Design and Formulation of Chemical Products from Icesi University (Cali, Colombia). Such anionic polymers were utilized as received. The phytic acid (PA) solution (MKCB0619V) was purchased from Sigma Aldrich Co (St. Louis, MO, United States). Sodium hydroxide (lot B1315798639) and acetic acid (lot K41575763) were obtained from Merck (Darmstadt, Germany). Ampicillin trihydrate (Amp) was provided by Tecnoquimicas S.A. (Cali, Colombia). Staphylococcus aureus strains (ATCC25923, ATCC29213 and ATCC43300) were obtained from Microbiologics Inc.© (St. Cloud, MN, USA) and were reconstituted according to the supplier instructions.
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Polymeric Salts for Antimicrobial Nanoparticles
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The polymeric salts of PMA-VP (MV: 25 kDa, Mw monomeric unit: 276 g/mol, ionization degree: >95%) and PMA-VP-N10 (MV: 25 kDa, Mw monomeric unit: 387 g/mol, functionalization degree: ~90%) were provided by the Laboratory of Design and Formulation of Chemical Products from Icesi University (Cali, Colombia), which were duly cryopreserved under vacuum at −5 °C. All data on the synthesis and characterization of these materials were previously reported [23 (link)]. However, to verify the polymer integrity, new IR spectra were taken on a FTIR spectrophotometer (Thermo Fisher Scientific Nicolet 6700) and compared with those taken after their synthesis. Tecnoquimicas S.A. Pharmaceutical Company (Cali, Colombia) provided the ampicillin (349 g/mol). Staphylococcus aureus ATCC25923, ATCC29213, and ATCC43300 were acquired from Microbiologics Inc.© (St. Cloud, MI, USA). The nanoparticle solutions were prepared using ultra-pure water, which was obtained from a purification system (Millipore Elix Essential, Merck KGraA, Darmstadt, Germany).
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Polymeric Salts for Antimicrobial Nanoparticles
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The polymeric salts of PAM-18Na (monomeric unit: 412 g/mol, Mn:30–50 kDa, ionization degree: 99%) and EuCl (monomeric unit: 435 g/mol, Mn: 47 kDa, ionization degree: 38%) were provided by the Laboratory of Design and Formulation of Chemical Products from Icesi University (Cali, Colombia) and they were used in the form that they were received. Likewise, all the data on the synthesis and characterization of these materials were previously reported [77 (link),78 (link)]. Tecnoquimicas S.A. Pharmaceutical Company (Cali, Colombia) provided the ampicillin. Staphylococcus aureus ATCC25923, ATCC29213, and ATCC43300 were purchased from Microbiologics Inc.© (St. Cloud, MI, USA) and were reconstituted according to the supplier guidelines. The nanoparticle suspension was mixed with ultra-pure water that was obtained from a purification system (Millipore Elix Essential, Merck KGraA, Darmstadt, Germany).
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Poly(maleic anhydride-alt-octadecene) Antimicrobial Assay
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Poly(maleic anhydride-alt-octadecene) denominated like PAM-18 with average Mw 30,000–50,000 and Lucifer yellow were obtained from Sigma-Aldrich®, ampicillin trihydrate—here referred to as AT—was from Fersinsa Gb® (Coahuila, Mexico), recombinant β-lactamase from Pseudomonas fluorescens was obtained from Sigma® (Saint Louis, MO, USA). It was received lyophilized and suspended according to manufacturer indications. Ultrapure water was obtained with an Elix Essential Millipore® (Darmstadt, Germany) purification system. All other reagents were from Merck® (Kenilworth, NJ, USA). Bacterial strains: S. aureus strains ATCC 25923, ATCC 29213 y ATCC 43300 were purchased from Microbiologics Inc.© (St Cloud, MN, USA) and were reconstituted according to the instructions.
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