Mda mb 435

Human melanoma cell line MDA-MB-435S was purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Cell lines were cultured in DMEM growth media (Life Technologies, Carlsbad, CA, USA) which was supplemented with 10% fetal bovine serum (FBS, Life Technologies) and maintained at 37°C in a humidified atmosphere at 5% CO₂. DCA and 2-MeOE2 were purchased from Sigma-Aldrich (St. Louis, MO, USA).

T47D, MCF7, ZR-75-1, BT474, MDA-MB-231, MDA-MB-435S, and BT549 cells were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). MCF-10A cells were obtained from American Tissue Culture Collection (ATCC, Manassas, VA, USA). T47D, ZR-75-1, BT474, MDA-MB-231, and BT549 cells were maintained in RPMI 1640 (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 IU ml⁻¹ penicillin, 100 μg ml⁻¹ streptomycin, and 2 mML-glutamine. MCF7 cells were maintained in DMEM (Gibco) supplemented with 10% FBS, 100 IU ml⁻¹ penicillin, 100 μg ml⁻¹ streptomycin, and 2 mML-glutamine; MDA-MB-435S cells were maintained in Leibovitz's L-15 (Gibco) supplemented with 10% FBS; MCF-10A cells were maintained in DMEM/F12 (1 : 1) (HyClone, Logan, UT, USA) supplemented with 5% horse serum (HyClone), 20 ng ml⁻¹ epidermal growth factor (EGF; Peprotech, Rocky Hill, NJ, USA), 100 ng ml⁻¹ cholera toxin (Sigma, St Louis, MO, USA), 10 μg ml⁻¹ insulin (Sigma), and 0.5 μg ml⁻¹ hydrocortisone (Sigma).
For EMT induction, cells were cultured in media with 50 ng ml⁻¹ IL-6 (Peprotech), 20 ng ml⁻¹ EGF (Peprotech) and 20 ng ml⁻¹ bFGF (Peprotech), or 10 ng ml⁻¹ transforming growth factor-β1 (TGF-β1; Peprotech) at 37 °C in 5% CO₂.