We used commercially available ELISA kits to analyze the CEA, CA125, CA242, and CA19-9 (Fujirebio Diagnostics, Gothenburg, Sweden) levels (Supplementary Table 2). We used the following reference values based on 97.5% percentiles defined from 100-210 healthy donors: 5 g/L for CEA, 39 U/mL for CA125, 29 U/mL for CA242, and 25 U/mL for CA19-9.
Ca125
Manufactured by Fujirebio
Sourced in Sweden
CA125 is a laboratory test used to measure the levels of a specific protein in the blood. This protein is associated with certain types of cancer, particularly ovarian cancer.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ca125
1
ELISA Analysis of Tumor Markers
2
Multiparametric Immunoassay Optimization
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The 96-well microtiter plates coated with streptavidin (SA plates, product #: 41-07TY), wash buffer (product #: 42-01TY), and RED assay buffer (product #: 42-02TY) were purchased from Kaivogen Oy (Turku, Finland). From R&D systems, we acquired the anti-integrin’s antibody and anti-tetraspanins’ antibody, e.g., anti-CD151 (Mab 1884). The anti-CD63 (H5C6 clone) antibody was purchased from BD Science (Vantaa, Finland). The plate washer (Delfia PlateWash 1296-026) and plate shaker (Delfia PlateWash 1296-026) were from Wallac Oy (Turku, Finland), and HIDEX™ fluorimeter was from HIDEX Oy (Turku, Finland). The reagents for cell line cultures were Gibco brand of Thermo Fisher Scientific (Waltham, MA, USA) except for glutamine, which was Ultraglutamine from Lonza (Basel, Switzerland), and phosphate-buffered saline, which was from GE Healthcare (Chicago, IL, USA). The conventional CEA, CA125, and CA19-9 EIA kits were kindly provided by Fujirebio Diagnostics.
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