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Jem 1010 80 kv

Manufactured by JEOL
Sourced in Japan

The JEM-1010 80 kV is a transmission electron microscope (TEM) produced by JEOL. It operates at an accelerating voltage of 80 kV and is designed to provide high-quality imaging of a wide range of samples.

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2 protocols using jem 1010 80 kv

1

Negative Staining for TEM Imaging

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Grids for transmission electron microscopy were prepared by negative staining, using 2% uranyl acetate solution 0.1 mg/mL protein in 1 M NaCl 20 mM Tris pH 7.5. Images were recorded at 120kx magnification, on a TEM JEOL JEM-1010 80 kV instrument.
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2

Characterization of AgNPs and AuNPs

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AgNPs (SKU: 576832) and AuNPs (SKU: GRF-60-20) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Cytodiagnostics (Burlington, ON, Canada), respectively. According to the manufacturer’s protocol, the AgNPs (<100 nm) were obtained using PVP as a dispersant with the purity of 99.5% trace metal basis. The reactant free AuNPs (60 nm) were stabilized using citric acid and dispersed in 0.1 mM phosphate buffered saline (PBS) with the purity of 99.0% reactant free. Both AgNPs and AuNPs suspensions were prepared by diluting the particles in deionized water. The hydrodynamic size distribution and zeta potential of the nanoparticles were measured using a dynamic light scattering (DLS) spectrometer (NanoZS, Malvern, UK). The morphology and size of the nanoparticles were analyzed by TEM (transmission electron microscopy, JEM1010-80 kV, JEOL, Japan). The silver nitrate (AgNO3, Sigma, St. Louis, MO, USA) and cadmium chloride (CdCl2, Sigma, St. Louis, MO, USA) were prepared by dissolving their respective powders in deionized water.
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