Samples for electron microscopy analysis were taken from the explanted valve leaflets and were rinsed in a solution of paraformaldehyde. For scanning electron microscopy (SEM), the tissue samples were dehydrated in a graded acetone series, and critical-point dried. Specimens were then glued to metal stubs, coated with gold, and examined in a Hitachi S-520 scanning electron microscope (Hitachi, Ltd., Chiyoda, Tokyo). For transmission electron microscope (TEM), the tissue samples were postfixed in osmium tetroxide and embedded in araldite. Ultrathin sections were stained with uranylacetate and lead citrate and observed with a Hitachi H-7650 transmission electron microscope (Hitachi, Ltd., Chiyoda, Tokyo).
S 520 scanning electron microscope
Manufactured by Hitachi
Sourced in Japan
The S-520 scanning electron microscope is a high-performance instrument designed for advanced microscopy applications. It utilizes a focused electron beam to scan the surface of a sample, allowing for detailed imaging and analysis at the nanometer scale. The S-520 provides high-resolution images, enabling users to examine the surface topography and microstructural features of a wide range of materials.
Automatically generated - may contain errors
Lab products found in correlation
H 7650 transmission electron microscope, by Hitachi (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Axioskop 40 fluorescence microscope, by Zeiss (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Ft ir spectra, by Bruker (1 mentions)
12 protocols using s 520 scanning electron microscope
1
Ultrastructural Analysis of Explanted Valve Leaflets
2
Spore Morphology and Germination Microscopy
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Morphological characteristics of spores upon germination were examined under a Axioskop 40 fluorescence microscope (Zeiss, Oberkochen, Germany) without or with staining using 0.2 μg·μL−1 4,6-diamidino-2-phenylindole (Molecular Probes, Carlsbad, USA). Observation of MS was performed under HITACHI S-520 scanning electron microscope (Hitachi, Tokyo, Japan) (Dai et al., 2002 ). Living MS were prepared by standard techniques for scanning electron microscope observation. Spores were fixed, gradually dehydrated, and critically point dried by Balt-Tec CPD-030 critical point dryer (Balt-Tec AG, Balzers, Liechtenstein) according to Dai et al. (2002 ). Dry spores were mounted on aluminum stubs using double-sided tape and coated with gold-palladium using a Denton Vacuum Desk II sputter coater (Denton Vacuum Inc., Cherry Hill, USA).
3
Scanning Electron Microscopy Sample Preparation
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Cells cultured on a coverslip were rinsed with ice‐cold 1× PBS (pH 7.2) and fixed with 2% glutaraldehyde for 3 hours at 4°C. After washing with 1× PBS, the cells were fixed with 1% OsO4 for 2 hours and dehydrated in ethanol. The samples were then critical‐point dried and sputter‐coated with gold. Samples were examined, and images were acquired using a HITACHI S‐520 scanning electron microscope.
4
Ex vivo expanded hDPSC sheet characterization
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Ex vivo expanded hDPSC sheets grown for 7–10 days were fixed using 2.5 % glutaraldehyde in 0.1 mol/l sodium cacodylate buffer (pH 7.2) for 2 h at 4 °C. After washing with sodium dimethylarsenate buffer, the cells were post-fixed in 1 % osmium tetroxide, dehydrated with gradient alcohol, and then incubated with isoamyl acetate. After gold coating, five samples were examined using a Hitachi S-520 scanning electron microscope (Hitachi, Tokyo, Japan).
5
Identification of Fe(III)-reducing and NH4+ Oxidizing Bacteria
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The 16S rRNA gene of all the isolated strains with both Fe(III) reduction and NH4+ oxidation activity in the liquid media were amplified via PCR using multiple bacterial universal primers (Table A in S1 File ), under the following conditions: 5 min at 94 °C, 30 cycles of 1 min at 94 °C, 1 min at specific annealing temp., 1.5 min at 72 °C and a step of 10 min at 72 °C. All the amplified PCR products were then run and visualized on a 1.2% agarose gel electrophoresis with syber safe stain in E-gel system. All the amplified products were purified and sequenced by Genewiz Inc. The sequences were analyzed using a BLAST search of the GenBank database. Phylogenetic trees of the partial 16S rRNA gene sequences and nearly full-length reference sequences were constructed using the MEGA software [12 (link)]. Selected strains were also settled on glass slides and viewed using a phase-contrast microscope (Leitz Labolux, ×400) and a Hitachi S-520 scanning electron microscope for morphological analysis [13 (link)].
6
Scanning Electron Microscopy of Neuronal Synapses
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The primary neurons were analyzed using a Hitachi S-520 scanning electron microscope to detect changes in the following surface morphological features: synaptic cleft (nm), synaptic interface curvature, presynaptic active zone length (nm), and synaptic PSD (nm). For standard error of the mean (SEM), living cells require chemical fixation to preserve and stabilize their structure. Fixation is performed by incubation in a solution of a buffered glutaraldehyde. Samples for imaging will be mounted on a solid flat substrate. Before observation, the microscope was checked to see if it was energized and ready for operation, and all required personal protective equipment was worn. The samples were placed inside the microscope, and following ion milling to expose the required region, an electron beam of 1.6 kV was used with a milling beam of 30 kV and 800 pA. Each milling and imaging cycle took ~ 90 sec, with a pixel dwell time of 10 μsec for the electron beam. Six hundred to a thousand images were collected by sequentially milling and imaging, with a final image size of 2048 by 1536 pixels. Each pixel was 5 × 5 nm. Images were obtained on the screen and saved in the appropriate folder.
7
Characterizing HDPE/PVA Film Morphology
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The distribution of PVA in the HDPE/PVA films was studied using a Hitachi S-520 scanning electron microscope (Tokyo, Japan). Prior to the SEM observations, the samples were fractured in liquid nitrogen. The fractured surfaces were decorated with gold using an Eiko IB-3 unit by ionic plasma deposition.
8
Ultrasonic-Assisted Extraction and HPLC Analysis
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An SHZ-D(III) circulating water vacuum (Yu Hua Instrument), a DA-3A ultrasonic extractor (Lingtong Electronics, Lecong, Shunde) and an LSP01-1A microsyringe pump (Longer Pump) were used. The Agilent 1200 high-performance liquid chromatograph includes a G1311A quaternary pump, G1316A thermostatted column, G1322A vacuum degasser, G1314B variable wavelength detector and G1328B manual injector (20 µl). The S-520 scanning electron microscope (SEM) is produced by Hitachi.
Melamine, ethylene glycol dimethacrylate (EDMA), methacrylic acid (MAA), azodiisobutyronitrile (AIBN) and other analytical reagents were purchased from the Aladdin Reagent Company (Shanghai, China). HPLC-grade methanol was supplied by Merck (chromatographically pure, Germany). Glass capillary (10 cm long, 500 µm i.d.) was purchased from the West China Center of Medical Sciences (China). Benzene, 1-dodecanol and other analytical reagents were purchased from the Guangzhou Chemical Reagents Factory (China), and eggs were purchased from local markets. After ultrasonic degassing and filtration through 0.22 µm membranes, all solutions were used for HPLC analysis.
Melamine, ethylene glycol dimethacrylate (EDMA), methacrylic acid (MAA), azodiisobutyronitrile (AIBN) and other analytical reagents were purchased from the Aladdin Reagent Company (Shanghai, China). HPLC-grade methanol was supplied by Merck (chromatographically pure, Germany). Glass capillary (10 cm long, 500 µm i.d.) was purchased from the West China Center of Medical Sciences (China). Benzene, 1-dodecanol and other analytical reagents were purchased from the Guangzhou Chemical Reagents Factory (China), and eggs were purchased from local markets. After ultrasonic degassing and filtration through 0.22 µm membranes, all solutions were used for HPLC analysis.
9
Characterization of Microcapsule Properties
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The mean size, size distribution, and the zeta potential of the OMC microcapsules were recorded by DLS with a Zetasizer Nano ZS (Malvern Instruments, Worcestershire, UK). The average diameters and size distributions were acquired by measuring 100 droplets individually (Image J software, National Institutes of Health, Bethesda, MD, USA), for which the model is expressed as:
The morphology of OMC microcapsules was observed by a Hitachi S-520 scanning electron microscope (SEM, Hitachi, Tokyo, Japan) with accelerating voltage of 15 kV. FTIR spectra (Bruker TENSOR II, Karlsruhe, Germany) were used to analyze the structure of the test samples in the wavelength range of 4000–400 cm−1 at 4.0 cm−1 resolution. The thermal stability of OMC, SC, and GA complex and OMC microcapsules was determined by using a NETZSCH STA 449C thermal analysis system (Selb, Germany).
The morphology of OMC microcapsules was observed by a Hitachi S-520 scanning electron microscope (SEM, Hitachi, Tokyo, Japan) with accelerating voltage of 15 kV. FTIR spectra (Bruker TENSOR II, Karlsruhe, Germany) were used to analyze the structure of the test samples in the wavelength range of 4000–400 cm−1 at 4.0 cm−1 resolution. The thermal stability of OMC, SC, and GA complex and OMC microcapsules was determined by using a NETZSCH STA 449C thermal analysis system (Selb, Germany).
10
Omental Tissue SEM Preparation
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Omental samples were collected, fixed overnight with 2.5% glutaraldehyde in 0.1 M PIPES buffer (pH 6.9), washed three times in fresh Pipes buffer (pH 6.9), and post-fixed for 1.5 hours in 1% osmium tetroxide in 0.1 M Pipes buffer (pH 6.9). The samples were washed in dH2O three times and then dehydrated in increasing concentrations of ethanol (50, 70, 90 and 100%). Specimens were critical point-dried from liquid carbon dioxide, coated to a thickness of 3 nm with an osmium plasma coater, and observed with a Hitachi S-520 scanning electron microscope.
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