The following primary antibodies were used for Western blots: GFP rabbit antibody (1:1000, #ab290, Abcam), CCDC170 rabbit antibody (1:500, #Ab97814, Abcam), RCAS1 rabbit antibody (1:100, #12290, Cell Signaling), acetylated α-tubulin mouse antibody (1:500, #sc-23950, Santa Cruz), α-tubulin mouse antibody (1:2000, #T5168, Sigma-Aldrich), and β-actin mouse monoclonal antibody (1:5000, Cat#A5316, Sigma-Aldrich). For secondary antibodies, ECL HRP-conjugated anti-mouse or anti-rabbit IgG (1:2000, GE Healthcare) were used.
Ecl hrp conjugated anti mouse or anti rabbit igg
Manufactured by GE Healthcare
Sourced in United States
The ECL HRP-conjugated anti-mouse or anti-rabbit IgG is a secondary antibody used in western blotting and other immunodetection techniques. It is conjugated with horseradish peroxidase (HRP), which enables the detection of target proteins through a chemiluminescent reaction.
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2 protocols using ecl hrp conjugated anti mouse or anti rabbit igg
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Western Blot Primary and Secondary Antibodies
2
Comprehensive Antibody Characterization
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The following primary antibodies were used: NF90 rabbit polyclonal antibody (1:10000, Cat# ab131004, Abcam, Cambridge, MA, USA), NF45 rabbit monoclonal antibody (1:1000, Cat# ab131004, Abcam), p21WAF1 mouse monoclonal antibody (1:200, Cat# OP64, Calbiochem, La Jolla, CA, USA), Keratin mouse monoclonal antibody (1:1000, Cat# ab8068, Abcam), vimentin mouse monoclonal antibody (1:1000, Cat# V5255, Sigma-Aldrich, St. Louis, MO, USA), and β-actin mouse monoclonal antibody (1:5000, Cat#A5316, Sigma-Aldrich). For secondary antibodies, ECL™ HRP-conjugated anti-mouse or anti-rabbit IgG (GE Healthcare, Chicago, IL, USA) were used. For pull-down western experiments, HRP-conjugated anti-rabbit heavy chain (Jackson ImmunoResearch Labs, West Grove, PA, USA) was used.
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