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Perfecta fastmix 2 low rox kit

Manufactured by Quanta Biosciences
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The PerfeCTa FastMix II Low ROX kit is a ready-to-use reaction mix for real-time quantitative PCR (qPCR) applications. It contains a proprietary buffer system, stabilizers, and a chemically-modified hot-start DNA polymerase for reliable and sensitive amplification of target sequences.

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Lab products found in correlation

Nuclisens easymag 2.0 instrument, by bioMérieux (2 mentions) Cfx96 touch thermocycler, by Bio-Rad (2 mentions)

2 protocols using perfecta fastmix 2 low rox kit

1

Quantifying Viral DNA in EV Fractions

Check if the same lab product or an alternative is used in the 5 most similar protocols
200 μl aliquots of EV fraction, intermediate fraction, and HCMV fraction were subjected to nucleic acid extraction using a NucliSENS easyMAG 2.0 instrument (BioMérieux, Durham, NC). Quantitative real-time PCR (qPCR) was performed with a PerfeCTa FastMix II Low ROX kit (Quanta BioSciences, Gaithersburg, MD) using the following primer set: HHV-5 FWD: AACCAAGATGCAGGTGATAGG, HHV-5 REV: AGCG TGACGTGCATAAAGA, and the probe: /56-FAM/TACCTGGAG/ZEN/ TCCTTCTGCGAGGA/3IABkFQ/. Amplifications were carried out on a BioRad CFX96 Touch Thermocycler according to the following cycling parameters: 2 min at 95 °C followed by 44 cycles of 10 s at 95 °C and 30 s at 60 °C.
Zicari S., Arakelyan A., Palomino R.A., Fitzgerald W., Vanpouille C., Lebedeva A., Schmitt A., Bomsel M., Britt W, & Margolis L. (2018). Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins. Virology, 524, 97-105.
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2

Quantifying Viral DNA in EV Fractions

Check if the same lab product or an alternative is used in the 5 most similar protocols
200 μl aliquots of EV fraction, intermediate fraction, and HCMV fraction were subjected to nucleic acid extraction using a NucliSENS easyMAG 2.0 instrument (BioMérieux, Durham, NC). Quantitative real-time PCR (qPCR) was performed with a PerfeCTa FastMix II Low ROX kit (Quanta BioSciences, Gaithersburg, MD) using the following primer set: HHV-5 FWD: AACCAAGATGCAGGTGATAGG, HHV-5 REV: AGCG TGACGTGCATAAAGA, and the probe: /56-FAM/TACCTGGAG/ZEN/ TCCTTCTGCGAGGA/3IABkFQ/. Amplifications were carried out on a BioRad CFX96 Touch Thermocycler according to the following cycling parameters: 2 min at 95 °C followed by 44 cycles of 10 s at 95 °C and 30 s at 60 °C.
Zicari S., Arakelyan A., Palomino R.A., Fitzgerald W., Vanpouille C., Lebedeva A., Schmitt A., Bomsel M., Britt W, & Margolis L. (2018). Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins. Virology, 524, 97-105.
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