Nav‐Gal was characterized by conventional techniques. For samples preparation, pure Nav‐Gal was dissolved in deuterochloroform (CDCl3; Sigma) and transferred to a Wilma NMR tube (Sigma). Samples were immediately analyzed by proton nuclear magnetic resonance (1H NMR), Carbon‐13 nuclear magnetic resonance (13C NMR) and homonuclear bidimensional correlated spectroscopy (COSY NMR). NMR spectra were acquired with a Bruker FT‐NMR Avance 400 (Ettlingen, Germany) at 300 K and analyzed with MestReNova 6.0 software. Chemical shifts are expressed as δH (ppm) relative tetramethylsilane (TMS). Attenuated total reflectance (ATR) was performed in a Bruker Tensor 27 FT‐IR (Ettlingen, Germany) at room temperature and analyzed with OPUS Data Collection Program (V 1.1). Finally, high resolution mass spectrometry (HRMS) was recorded with an ABSciex TRIPLETOF T5600.
Ft nmr avance 400
Manufactured by Bruker
Sourced in Germany
The FT-NMR Avance 400 is a nuclear magnetic resonance (NMR) spectrometer manufactured by Bruker. It operates at a frequency of 400 MHz and is designed for analytical applications in various fields, including chemistry, materials science, and life sciences.
Automatically generated - may contain errors
Lab products found in correlation
Tripletoft5600, by AB Sciex (2 mentions)
Tensor 27 ftir, by Bruker (1 mentions)
Balb cbyj female mice, by Charles River Laboratories (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tcs sp8 aobs, by Leica (1 mentions)
Victor multilabel plate reader, by PerkinElmer (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Fv1000mpe confocal microscope, by Olympus (1 mentions)
Palbociclib, by Selleck Chemicals (1 mentions)
Topspin 3, by Bruker (1 mentions)
Avance 400 mhz spectrometer, by Bruker (1 mentions)
Topspin 1, by Bruker (1 mentions)
4 protocols using ft nmr avance 400
1
Characterization of Nav-Gal by NMR and IR
2
Palbociclib Anti-Cancer Compound Characterization
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All chemical reagents
were purchased from
Sigma–Aldrich, while anhydrous solvents and phosphate-buffered
saline (PBS, 0.01 M) were purchased from Scharlab S.L. and used without
further purification. Palbociclib was purchased from Selleckchem,
and Dulbecco’s modified Eagle medium (DMEM) and fetal bovine
serum (FBS) were purchased from Gibco. Flat-bottom clear 96-well plates
were purchased from Promega. High-resolution mass spectrometry (HRMS)
and the data was recorded with a TripleTOF T5600 (ABSciex, U.S.A.)
spectrometer. 1H and 13C NMR spectra were collected
on a Bruker FT-NMR Avance 400 (Ettlingen, Germany) spectrometer at
300 K, using TMS as an internal standard. HPLC measures were obtained
by a Waters 1525 binary HPLC pump, and spectra were recorded by a
Waters 2998 photodiode array at 260 nm. Fluorescence spectra were
recorded by a JASCO FP-8500 fluorescence spectrophotometer, Luminescence
was collected in a VICTOR multilabel plate reader (PerkinElmer). Confocal
fluorescence images were taken on a Leica TCS SP8 AOBS, and two-photon
images were acquired using a multiphoton Olympus FV1000MPE confocal
microscope. Images were analyzed using ImageJ software. The SK-Mel-103
(human melanoma) cancer cell line and 4 T1 (breast cancer cells) were
acquired from the American Type Culture Collection (ATCC). BALB/cByJ
female mice were purchased from Charles River laboratories, France.
were purchased from
Sigma–Aldrich, while anhydrous solvents and phosphate-buffered
saline (PBS, 0.01 M) were purchased from Scharlab S.L. and used without
further purification. Palbociclib was purchased from Selleckchem,
and Dulbecco’s modified Eagle medium (DMEM) and fetal bovine
serum (FBS) were purchased from Gibco. Flat-bottom clear 96-well plates
were purchased from Promega. High-resolution mass spectrometry (HRMS)
and the data was recorded with a TripleTOF T5600 (ABSciex, U.S.A.)
spectrometer. 1H and 13C NMR spectra were collected
on a Bruker FT-NMR Avance 400 (Ettlingen, Germany) spectrometer at
300 K, using TMS as an internal standard. HPLC measures were obtained
by a Waters 1525 binary HPLC pump, and spectra were recorded by a
Waters 2998 photodiode array at 260 nm. Fluorescence spectra were
recorded by a JASCO FP-8500 fluorescence spectrophotometer, Luminescence
was collected in a VICTOR multilabel plate reader (PerkinElmer). Confocal
fluorescence images were taken on a Leica TCS SP8 AOBS, and two-photon
images were acquired using a multiphoton Olympus FV1000MPE confocal
microscope. Images were analyzed using ImageJ software. The SK-Mel-103
(human melanoma) cancer cell line and 4 T1 (breast cancer cells) were
acquired from the American Type Culture Collection (ATCC). BALB/cByJ
female mice were purchased from Charles River laboratories, France.
3
Spectroscopic Characterization of Compounds
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NMR spectra (1H-NMR, 13C-NMR, DEPT135, COSY, HMBC, and HSQC) were measured on a BRUKER AVANCE 400 FT-NMR or a BRUKER AVANCE DMX 600 FT-NMR spectrometer at 25 °C. Proton chemical shifts (δ scale) are expressed in parts per million (ppm) and were determined relative to a residual protic solvent as an internal reference (CDCl3: δ = 7.26 ppm, MeOD: δ = 3.31 ppm). Data for 1H-NMR spectra are listed as follows: chemical shift (δ ppm) (multiplicity, integration, coupling constants (Hz), and assigned protons). Couplings are indicated as: s = singlet, d = doublet, t = triplet, and m = multiplet. Carbon nuclear magnetic resonance (13C-NMR) spectra were recorded with the same BRUKER spectrometers at 100.9 and 150.9 MHz, respectively. Carbon chemical shifts (δ scale) are indicated in ppm and calibrated to the carbon resonance of the respective solvent (CDCl3: δ = 77.16 ppm, MeOD: δ = 49.00 ppm, DMSO-d6: δ = 2.50 ppm) (ESI, Fig. S8–S11 and Table S2† ).
4
Solid-state NMR Analysis of Bamboo Composition
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Solid-state NMR (nuclear magnetic resonance) spectra were obtained from Bruker Avance 400 FT-NMR (USA), as described previously [18, 19] . All the 13 C CP MAS NMR (cross polarization and magic angle spinning NMR) measurements were performed with a Bruker Avance 400 MHz spectrometer operating at 100.62 MHz at room temperature. About 90 mg of dried bamboo powders were filled in cylindrical rotors made of zirconia (outer diameter 4 mm) with kel-F end caps. All 13 C NMR spectra were collected by the ramped CP pulse program [20] . The magic angle spinning speed was 13,000 Hz, acquisition time 10.3 ms, and delay between pulses 5 s with contact times of 1 ms, respectively. For each spectrum, 1000-2000 scans were collected. The chemical shifts in 13 C NMR spectra were referenced using the adamantine as an internal standard (38.56 ppm). The 1 H radio-frequency field strength was set to give 90°pulse duration around 4.0 ls. NMR data were collected by the Topspin 1.3 software (Bruker Co.), and the regional integration values were obtained using the TopSpin 3.0 software. For FT-IR (Fourier transform infra-red) spectra, about 100 lm thick veneers collected from the bamboo surface were milled and mixed with KBr. FT-IR spectra were obtained from Perkin-Elmer 1800 spectrometer (USA).
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