Adult trachea tissue was fixed using Karnovsky's fixative in 0.1 M sodium phosphate buffer (Sorenson's), washed with Sorenson's sodium phosphate buffer and post-fixed using 1% OsO4 in Sorenson's for 1 hour. Tissue was dehydrated by passaging through a graded series of ethanol solutions, then critical point dried using a Tousimis 931 Super Critical Point Dryer. The tissue was mounted on aluminum stubs and sputter coated with gold using a PELCO SC-7 coater. The samples were viewed on an FEI XL30 TMP SEM and digital images were collected. SEM was performed in the electron microscopy facility of the University of California at Davis.
931 super critical point dryer
Manufactured by Tousimis
The 931 Super Critical Point Dryer is a laboratory equipment designed for critical point drying of samples. It provides a controlled environment for the transition of a substance from its liquid state to its gaseous state without passing through the intermediate liquid-gas phase boundary.
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Lab products found in correlation
2 protocols using 931 super critical point dryer
1
Scanning Electron Microscopy of Trachea
2
Scanning Electron Microscopy of Trachea
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Adult trachea tissue was fixed using Karnovsky's fixative in 0.1 M sodium phosphate buffer (Sorenson's), washed with Sorenson's sodium phosphate buffer and post-fixed using 1% OsO4 in Sorenson's for 1 hour. Tissue was dehydrated by passaging through a graded series of ethanol solutions, then critical point dried using a Tousimis 931 Super Critical Point Dryer. The tissue was mounted on aluminum stubs and sputter coated with gold using a PELCO SC-7 coater. The samples were viewed on an FEI XL30 TMP SEM and digital images were collected. SEM was performed in the electron microscopy facility of the University of California at Davis.
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