ChIP assay was performed using the SimpleChIP® Enzymatic Chromatin IP kit (Agarose Beads) (Cell Signaling #9002). The general procedure for ChIP assay was followed and is similar to that reported previously (Caslini et al., 2006 (link)). Chromatin was immunoprecipitated by incubating with antibodies to GATA6 (R & D systems, AF1700) or GATA4 (Santa Cruz Biotechnology, sc-9053), at 4 °C overnight. Mouse IgG antibodies were used as a negative control. For quantitative ChIP-qPCR, total inputs served as internal controls. EpiTect ChIP qPCR Primer Assay kit (QIAGEN, Cat# GPM1033446(+)01A) was used to for the amplification of Gata6 gene promoter of a 1 kb fragment starting at position −371.
Epitect chip qpcr primer assay kit
Manufactured by Qiagen
The EpiTect ChIP qPCR Primer Assay kit is a set of primers designed for quantitative real-time PCR (qPCR) analysis of chromatin immunoprecipitation (ChIP) samples. The kit provides pre-designed, validated primer pairs for specific genomic regions, enabling efficient and reliable quantification of DNA enrichment following ChIP.
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Lab products found in correlation
2 protocols using epitect chip qpcr primer assay kit
1
ChIP-qPCR of GATA6 and GATA4
2
ChIP-qPCR of GATA6 and GATA4
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